This study aims to determine the clinical symptoms and survival of white vaname shrimp in the prevention of Vibrio alginolyticus infection using miana leaf extract. The study consisted of 4 treatments and 3 replications. The treatments in this study were 1 treatment and 3 treatments using different concentrations of bacteria, namely 103 CFU/ml, 105 CFU/ml, and 107 CFU/ml. The research was carried out in June-July 2020 at the Hatchery Laboratory of the Faculty of Marine and Fisheries Sciences, Hasanuddin University. Data analysis used descriptive analysis and analysis of variance (ANOVA). The results showed that A (control) shrimp was under normal conditions, treatment B (103 CFU/ml) was red, treated C (105 CFU/ml) was red and had necrosis of the body, and treatment D (107 CFU/ml) was shrimp. reddening, necrosis of tail and body segments. The results of data analysis showed that the use of miana leaf extract had a significant effect (p<0.05) on the use of live vaname shrimp. The highest vaname shrimp survival was treatment A (control) at 100%, treatment B (103 CFU/ml) at 90%, treatment C (105 CFU/ml) at 63.33% and treatment D (107 CFU/ml) by 40%.
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