Insect herbivores are frequently reported to metabolize plant defense compounds, but the physiological and ecological consequences are not fully understood. It has rarely been studied whether such metabolism is genuinely beneficial to the insect, and whether there are any effects on higher trophic levels. Here, we manipulated the detoxification of plant defenses in the herbivorous pest diamondback moth (Plutella xylostella) to evaluate changes in fitness, and additionally examined the effects on a predatory lacewing (Chrysoperla carnea). Silencing glucosinolate sulfatase genes resulted in the systemic accumulation of toxic isothiocyanates in P. xylostella larvae, impairing larval development and adult reproduction. The predatory lacewing C. carnea, however, efficiently degraded ingested isothiocyanates via a general conjugation pathway, with no negative effects on survival, reproduction, or even prey preference. These results illustrate how plant defenses and their detoxification strongly influence herbivore fitness but might only subtly affect a third trophic level.

Multitrophic interactions involving plants, insect herbivores, and their antagonists are ubiquitous in terrestrial ecosystems and underpin our understanding of the structure and function of ecological communities (Stam et al., 2014). Most plants in nature are attacked by insect herbivores, and high infestations can severely damage plant tissues and thus reduce plant fitness (Johnson, Lajeunesse, & Agrawal, 2006). To prevent or reduce attack, plants employ an array of strategies to reduce herbivory, including the production of a wide assortment of toxic, repellent, antidigestive, and antinutritive chemical defences (Mithöfer & Boland, 2012). Plant chemical defences can also traverse trophic levels, moving up the food chain to affect not only the consuming herbivores but subsequently also herbivore predators (Hartmann, 2004;

The cabbage aphid Brevicoryne brassicae is a notorious agricultural pest that specializes on plants of the Brassicaceae family, which are chemically defended by glucosinolates. By sequestering glucosinolates from its host plants and producing its own activating enzyme (myrosinase), this aphid employs a self-defense system against enemies paralleling that in plants. However, we know little about the metabolic fate of individual glucosinolates during aphid sequestration and activation and about the biochemical effects of this defense on aphid enemies. Here, we probed these questions focusing on B. brassicae and a predatory lacewing, Chrysoperla carnea. We found that distinct glucosinolates were accumulated by B. brassicae at different rates, with aliphatic glucosinolates being taken up more quickly than indolic ones. B. brassicae myrosinase enzymatic activities toward different glucosinolates were strongly correlated to their rates of accumulation in vivo. Surprisingly, after simulated predation, the production of toxic isothiocyanate products (ITCs) was quantitatively outweighed by less toxic products such as nitriles and ITC-conjugates. Nevertheless, the defensive cocktails significantly impaired C. carnea development. Tissue-specific quantification of glucosinolate metabolites revealed that the lacewings employ both conjugation and mobilization to reduce the toxicity of aliphatic ITCs, but these strategies were only partially effective. These results clarify the metabolic fates of glucosinolates after sequestration by an aphid herbivore and further in a higher trophic level, as well as the consequences for predator survival and development, and might be instructive for integrative pest management approaches targeting the cabbage aphid.

We propose a new structure, one plate with a hole above the ellipsoid and the other plate with a hole below the ellipsoid, to obtain a repulsive Casimir force. The force was obtained numerically by using the in-house FDTD method, based on Maxwell’s stress tensor and harmonic expansion. The code can be verified by calculating the force of a perfect-metal ellipsoid centered above a perfect-metal plate with a hole. Our numerical method can effectively simulate the Casimir force by reducing the total simulated time. The further numerical results of realistic dielectric material immersing in fluids or adding other plates above the ellipsoid are also presented. It is not surprising to find that the larger differences can be achieved by varying the parameters such as the center-center separation, medium immersed, and the dielectric material of the structure. Thus, it is possible to tune these parameters relatively in the realistic microelectromechanical systems to overcome stiction and friction problems.

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