The interaction of pinostrobin (PS), a multitherapeutic agent with serum albumins of
various mammalian species namely, goat, bovine, human, porcine, rabbit, sheep and dog was
investigated using fluorescence quench titration and competitive drug displacement
experiments. Analysis of the intrinsic fluorescence quenching data revealed values of the
association constant, Ka in the range of 1.49 – 6.12 ×
104 M−1, with 1:1 binding stoichiometry. Based on the PS–albumin
binding characteristics, these albumins were grouped into two classes. Ligand displacement
studies using warfarin as the site I marker ligand correlated well with the binding data.
Albumins from goat and bovine were found to be closely similar to human albumin on the
basis of PS binding characteristics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.