Magnetic beads and magnetic Raman technology substrates have good magnetic response ability and surface-enhanced Raman technology (SERS) activity. Therefore, magnetic beads exhibit high sensitivity in SERS detection. In this paper, DNA cycle hybridization and magnetic bead models are combined to solve 0-1 integer programming problems. First, the model maps the variables to DNA strands with hairpin structures and weights them by the number of hairpin DNA strands. This result can be displayed by the specific binding of streptavidin and biotin. Second, the constraint condition of the 0-1 integer programming problem can be accomplished by detecting the signal intensity of the biological barcode to find the optimal solution. Finally, this model can be used to solve the general 0-1 integer programming problem and has more extensive applications than the previous DNA computing model.
DNA logic gates are an important branch of DNA computing and have a wide range of applications in DNA computing. In this study, logic circuits of AND gate and NAND gate are built on origami substrate. The realization of AND gate uses polymerase strand displacement (PSD) reaction and hybridization chain reaction (HCR). If there is a fluorescent band “1” displayed, the result is true. The realization of the NAND gate requires a cyclic reaction. If there is a fluorescent band “A” or “T” displayed, the result is true; if no fluorescent band is displayed, the result is false.
A Rh2(esp)2-catalyzed diastereoselective synthesis of highly functionalized β-lactams is developed. By employing Rh2(esp)2 as the catalyst, a reaction cascade operates by reducing an N-methyl nitrone to the corresponding N-methyl imine in the presence of a first molecule of the diazoacetoacetate enone. A second molecule of the diazoacetoacetate enone sensing a change in the reaction medium undergoes a Wolff rearrangement to afford a vinyl ketene. This vinyl ketene then reacts with the in situ generated N-methyl imine to diastereoselectively furnish a β-lactam with two contiguous stereogenic centers. Extension of this method to the [2+2]-cycloaddition of PMB-protected imines and diazoacetoacetate enones catalyzed by Rh2(esp)2 is also reported.
In recent years, in order to provide theoretical and scientific basis for the protection and rational utilization of plant resources in Lon longquan mountain gquan Mountain, the seed plants in Longquan Mountain were taken as the research object, and the field investigation was carried out by using the method of line plus typical sample plot, so as to further study the characteristics of investigated area were further studied. The results show: There are 231 species of 191 genus in 90 families, including 5 families, 9 genus and 11 species of gymnosperms, and 82 species of 182 genus and 85 species of angiosperms. The 14 distribution types of seed plants belong to the statistics: North temperate distribution There are 72 genus, accounting for 37.7% of the total genus of the area, reflecting the obvious north temperate nature of the flora. In addition, there are many ancient taxa and Cretaceous and Tertiary relict plants. There are 163 genus of single genus and genus genus, accounting for 85.3% of the total genus of the region, indicating that the plant origin is relatively old, there are 7 genus in China, which indicates that the unique phenomenon exists, but it is not obvious.
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