In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 ℃. Response surface methodology (RSM) were used to optimize the proportional composition of low-temperature phenoldegrading microbiota. Degradation of phenol below 160 mg L -1 by low-temperature phenol-degrading microbiota followed rst-order degradation kinetics. When the phenol concentration was greater than 200 mg L -1 , the overall degradation trend was in accordance with the modi ed Gompertz model. The experiments showed that the bacterial agent (three strains of low-temperature phenol-degrading bacteria were fermented separately and constructed in the optimal ratio) degraded phenol at the fastest rate. The above construction method is more advantageous in the actual wastewater treatment.
In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 ℃. Response surface methodology (RSM) were used to optimize the proportional composition of low-temperature phenol-degrading microbiota. Degradation of phenol below 160 mg L-1 by low-temperature phenol-degrading microbiota followed first-order degradation kinetics. When the phenol concentration was greater than 200 mg L-1, the overall degradation trend was in accordance with the modified Gompertz model. The experiments showed that the bacterial agent (three strains of low-temperature phenol-degrading bacteria were fermented separately and constructed in the optimal ratio) degraded phenol at the fastest rate. The above construction method is more advantageous in the actual wastewater treatment.
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