Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5- cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).
The influences of extract from Lactarius vellereus fermentation liquid on antioxidant enzymes activity of Alternaria alternata K. were evaluated. Results showed that, in treatment group, the activities of SOD, CAT and POD in mycelia of A. alternata increased at prophase, and SOD activity increased to maximum (31.562 U·g-1) at 5h, while both CAT and POD activity increased to maximum (0.041U·g-1·s-1 and 5.531U·g-1·min-1, respectively) after being administered with 10h. The activities of these three enzymes treated with extract decreased rapidly at anaphase and down to the minimum (0.828 U·g-1, 0.012 U·g-1·s-1, and 0 U·g-1·min-1, respectively) at 40h. While, in control group, SOD, CAT and POD activity always increased with treatment time prolonged. The activity of SOD, CAT and POD was affected byLactarius vellereus fermentation liquid extract so that it led to dysfunction of the free radical scavenging system. This might be one of the mechanisms that the extract inhibits the growth of A. alternata.
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