Colorimetric lateral flow immunoassay (LFIA) with gold nanoparticles (AuNPs) as signal reporters has been widely used in point-of-care testing. Nonetheless, the potential of traditional AuNP-based LFIA for the early diagnosis of disease is often compromised by limited sensitivity due to the insufficient colorimetric signal brightness of AuNPs. Herein, we develop a "three-in-one" multifunctional catalytic colorimetric nanohybrid (Fe 3 O 4 @MOF@Pt) composed of Fe 3 O 4 nanoparticles, MIL-100(Fe), and platinum (Pt) nanoparticles. Fe 3 O 4 @MOF@Pt displays enhanced colorimetric signal brightness, fast magnetic response, and ultrahigh peroxidasemimicking activity, which are beneficial to the enhancement of the sensitivity of LFIA by coupling with magnetic separation and catalytic amplification. When integrated with the dual-antibody sandwich LFIA platform, the developed Fe 3 O 4 @MOF@Pt can achieve an ultrasensitive immunochromatographic assay of procalcitonin with a sensitivity of 0.5 pg mL −1 , which is approximately 2280-fold higher than that of conventional AuNP-based LFIA and superior to previously published immunoassays. Therefore, this work suggests that the proposed catalytic colorimetric nanohybrid can act as promising signal reporters to enable ultrasensitive immunochromatographic disease diagnostics.
MicroRNAs (miRNAs) play important roles in post-transcriptional gene silencing by directing target mRNA cleavage or translational inhibition. Currently, hundreds of miRNAs have been identified in plants, but no report has been published of wild soybean (Glycine soja Sieb). We constructed a small-RNA library consisting of 2 880 sequences with high quality, in which 1 347 were 19-24 nt in length. By utilizing the miRNA, Rfam and domesticated soybean expressed sequence tag database, we have analyzed and predicted the secondary structure of these small RNAs. As a result, 15 conserved miRNA candidates belonging to eight different families and nine novel miRNA candidates comprising eight families were identified in wild soybean seedlings. All these miRNA candidates were validated by northern blot and the novel candidates expressed in a tissue-specific manner. Furthermore, putative target genes were predicted for novel miRNA candidates and two of them were verified by 5 -rapid amplification of cDNA ends experiments. These results provided useful information for miRNA research in wild soybean and plants.
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