Abstract:In the present paper lipase catalyzed synthesis of Diacylglycerol (DAG) and Monoacylglycerol (MAG) by esterification of glycerol and Oleic acid in an organic solvent was studied, and optimal conditions for acylglycerols synthesis by enzyme were established. Suitable biocatalysts were chosen on the basis of systematic screening experiments regarding their regioselectivity, substrate tolerance. Lipases investigated were Rhizomucor miehei (RMIM) and Thermomyces lanuginosa (TLIM), which gave highest degree of esterification. The selective enzymatic production of Mono and Di-Olein (MO, DO) was optimized to obtain maximum yields. Hexane was useful organic solvent in glycerides synthesis. Based on this experiment, acylglycerols were synthesized on a preparative scale in good yields ( 90%).
Awareness regarding the harmful effects of excessive oils and fats intake is universal. Consequently, health conscious individuals are modifying their dietary habits and eating less fat , as high fat intake specially saturated fat is associated with increased risk for obesity, high blood cholesterol, coronary heart disease, cancer, and arteriosclerosis. Although oils and fats are not only the concentrated source of energy but also the source of essential fatty acids (EFA). Taking into account the drawbacks and benefits of oils and fats, the production of Structured Lipids (SLs) containing especial fatty acids has been now a days attracting more and more attention.
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