The outbreaks of pseudorabies have been frequently reported in Bartha-K61-vaccinated farms in China since 2011. To study the pathogenicity and evolution of the circulating pseudorabies viruses in Fujian Province, mainland China, we isolated and sequenced the whole genome of a wild-type pseudorabies virus strain named “FJ-2012.” We then conducted a few downstream bioinformatics analyses including phylogenetic analysis and pathogenic analysis and used the virus to infect 6 pseudorabies virus-free piglets. FJ-2012-infected piglets developed symptoms like high body temperature and central nervous system disorders and had high mortality rate. In addition, we identified typical micropathological changes such as multiple gross lesions in infected piglets through pathological analysis and conclude that the FJ-2012 genome is significantly different from known pseudorabies viruses, in which insertions, deletions, and substitutions are observed in multiple immune and virulence genes. In summary, this study shed lights on the molecular basis of the prevalence and pathology of the pseudorabies virus strain FJ-2012. The genome of FJ-2012 could be used as a reference to study the evolution of pseudorabies viruses, which is critical to the vaccine development of new emerging pseudorabies viruses.
We isolated an influenza strain named A/Swine/Fujian/F1/2010 (H1N2) from a pig suspected to be infected with swine flu. The results of electron microscopy, hemagglutination (HA) assay, hemagglutination inhibition (HI) assay, and whole genome sequencing analysis suggest that it was a reassortant virus of swine (H1N1 subtype), human (H3N2 subtype), and avian influenza viruses. To further study the genetic evolution of A/Swine/Fujian/F1/2010 (H1N2), we cloned its whole genome fragments using RT-PCR and performed phylogenetic analysis on the eight genes. As a result, the nucleotide sequences of HA, NA, PB1, PA, PB2, NP, M, and NS gene are similar to those of A/Swine/Shanghai/1/2007(H1N2) with identity of 98.9%, 98.9%, 99.0%, 98.6%, 99.0%, 98.9%, 99.3%, and 99.3%, respectively. Similar to A/Swine/Shanghai/1/2007(H1N2), we inferred that the HA, NP, M, and NS gene fragments of A/Swine/Fujian/F1/2010 (H1N2) strain were derived from classical swine influenza H3N2 subtype, NA and PB1 were derived from human swine influenza H3N2 subtype, and PB2 and PA genes were derived from avian influenza virus. This further validates the role of swine as a “mixer” for influenza viruses.
An emerging pseudorabies virus (PRV) variant has been reported on Bartha-K61-vaccinated farms since 2011, causing great economic losses to China’s swine-feeding industry. In this study, two vaccines, FJ-2012ΔgE/gI-GEL02 and FJ-2012ΔgE/gI-206VG, were administered to piglets for immune efficacy investigation. Humoral immunity response, clinical signs, survival rate, tissue viral load, and pathology were assessed in piglets. The results showed that both vaccines were effective against the PRV FJ-2012 challenge, the piglets all survived while developing a high level of gB-specific antibody and neutralizing antibody, the virus load in tissue was alleviated, and no clinical PR signs or pathological lesions were displayed. In the unimmunized challenged group, typical clinical signs of pseudorabies were observed, and the piglets all died at 7 days post-challenge. Compared with commercial vaccines, the Bartha-K61 vaccine group could not provide full protection, which might be due to a lower vaccine dose; the inactivated vaccine vPRV* group piglets survived, displaying mild clinical signs. The asterisk denotes inactivation. These results indicate that FJ-2012ΔgE/gI-GEL02 and FJ-2012ΔgE/gI-206VG were effective and could be promising vaccines to control or eradicate the new PRV epidemic in China.
LJ: Classification tree method for determining factors that affecting hatchability in chukar partridge (Alectoris chukar) eggs. Kafkas Univ Vet Fak Derg, 24 (3): 473-477, 2018473-477, . DOI: 10.9775/kvfd.2017 AbstractIn this study, we sequenced and analyzed the complete genomes of the porcine parvovirus 7 (PPV7) isolated in Fujian, Southeast China. Genomic comparison revealed that PPV7 isolates in Fujian shared higher (more than 98.5%) nucleotide homology and closer relationship with other PPV7 strains. Phylogenetic analysis based on NS indicated that PPV7 formed a distinct cluster (proposed Chapparvovirus) closer to subfamily Parvovirinae. However, phylogenetic analysis based on genome and VP showed that PPV7 isolates formed a distinct cluster closer to subfamily Densovirinae. No recombination event observed suggesting recombination did not contribution to the genetic diversity of PPV7. These findings demonstrated that PPV7 continuous circulating in China, which may help us to understand the evolution diversity of porcine parvoviruses.
Study on the antibody level differences of PED IgG and IgA, TGE IgG and PoR IgG after immunization with different porcine viral diarrhea vaccine combinations. Kafkas Univ Vet Fak Derg, 25 (5): 589-596, 2019. AbstractTo prevent porcine viral diarrhea, a vaccine combination that can provide good antibody levels needs to be determined. In this study, we screened 30 pregnant sows divided into six experimental groups, namely, five immunized groups and one control group, to investigate the antibody level differences of different vaccine combinations on porcine epidemic diarrhea (PED), porcine transmissible gastroenteritis (TGE), porcine rotavirus (PoR) IgG, and PED IgA. The antibody level was detected by ELISA. Results showed that the antibody levels of PED and TGE IgG in serum and PED IgA in breast milk of the "PT+PT*" vaccine combination group were higher than those of the other groups, and vaccine combination including "PTR" could stimulate the sows to produce PoR IgG antibody. These findings revealed that the vaccine combination of "PT+PT*" is optimal for preventing porcine viral diarrhea, and "PTR+PT*" may be an alternative option in areas under PoRV infection risk. This study suggested that pig farms should select suitable immunization on the basis of the local epidemic situation of porcine viral diarrhea.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.