Background: Mucin 1 (MUC1) is overexpressed in almost 90% of CA breast cases and is usually associated with poor prognosis. On the other hand, HER2 is profoundly expressed in breast carcinoma and is also linked with increased disease recurrence, tumor invasion and poor prognosis. Thus, this study was designed to assess the expression of MUC1 and HER2 against the histological grades of breast carcinoma in our population.
Materials and methods: It was a cross sectional analytical study conducted at Post Graduate Medical Institute/General Surgery Departments of Lahore General Hospital (LGH), Lahore. Duration of study was 12 month from 27-3-2018 to 30-3-2019. Total 60 newly diagnosed cases of CA breast were included in the study. Immunohistochemistry technique was used and MUC1 and HER2 markers were applied to check the probable association between the two markers and the histological grades of cancer.
Results: A total of 60 cases were included in the study, out of which 96.7% (n=58) were invasive ductal carcinoma, while 3.3% (n=2) were Invasive lobular carcinoma. Among the total, 60% (n=36) were found positive for MUC1 (moderate/strong) and 40% (n=24) were found negative (negative/weak). Regarding HER2, 26.7% (n=16) were strong positive, 20% (n=12) were at the borderline/equivocal and 53.3% (n=32) were found negative for HER 2.
Conclusion: Statistically significant association was found between MUC1 and HER2 against the histological grades of breast cancer patients (p <0.05).
Background: Malignant salivary gland tumors (MSGTs) consist of a heterogeneous group of neoplasms with complex clinicopathological features and biological behaviors. The purpose of this study was to determine the expression of Bcl-2 antiapoptotic protein in mucoepidermoid carcinoma (MEC), adenoid cystic carcinoma (ADCC), acinic cell carcinoma (ACC) and polymorphous low-grade adenocarcinoma (PLGA) of salivary glands and to find out its association with different grades of these tumors.
Material and Methods: This descriptive study included 55 cases of MSGTs. Tissue sections were stained with routine hematoxylin and eosin stain as well as Bcl-2 immunostain. MSGTs were graded as low grade (Low grade MEC, ACC, PLGA, and tubular pattern of ADCC), intermediate grade (cribriform pattern of ADCC, and intermediate grade of MEC) and high grade (high grade of MEC and solid pattern of ADCC) tumors on H&E sections. Bcl-2 expression was scored as ‘negative’ (<5% of neoplastic cells), ‘1’ (5-19% of neoplastic cells), ‘2’ (20-49% of neoplastic cells), and ‘3’ (>50% of neoplastic cells), respectively.
Results: MSGTs most commonly involved the parotid gland (52.7%), while ADCC (40%) and MEC (38.2%) were the most common tumors. Expression of Bcl-2 was strongly positive in 56.4% cases of MSGTs which included ADCC (71%), MEC (19.4%) and ACC (9.7%), respectively. A significant association was found between Bcl-2 staining and types of MSGTs i.e., MEC, ADCC, ACC (P = .001) as well as between Bcl-2 staining and grades of MSGTs (P = .013).
Conclusions: Bcl-2 protein is expressed in malignant salivary gland tumors. Its expression maybe helpful in grading small biopsies, predicting behavior, and planning targeted therapy of MSGTs.
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