The study was conducted at Kenya Agricultural and Livestock Research Organisation, Katumani, Machakos to evaluate the herbage quality of Brachiaria grass cultivars in semi arid regions of Eastern Kenya. Brachiaria cultivars namely B. decumbens cv. Basilisk, Brachiaria hybrid Mulato II, four Brachiaria brizantha cultivars Marandu, Xaraes, Piata, MG4 and Brachiaria humidicola cv Llanero were assessed with reference to their chemical and nutritive composition at 22, 24 and 28 weeks post seedling emergence. Rhodes grass (Chloris gayana cv KATR3) and Napier grass (P. pupureum cv. Kakamega I) were included as controls. There were significant differences (p<0.05) among the cultivars for crude protein, crude fibre (NDF, ADF and ADL) Ash, Calcium, Phosphorus, Dry Matter Digestibilty and Metabolisable energy during the the week 22 and 24 post seedling emergence harvest. At week 28 harvest interval, however, ADL values for all the cultivars were similar and only Marandu was significantly different (p<0.05) in terms of metabolizable energy from the rest. The mean CP content of the grasses decreased from 11.1% at week 22 to 6.3% at harvest week 28 harvest intervals. MG4, Mulato II and Xaraes were the only grasses able to meet minimum CP (7.0%) requirement for rumen microbial function throughout the harvest period. Ash and phosphorus values decreased whereas Calcium content increased for all the cultivars during the harvest intervals, with Mulato II recording the highest ash (15%) content during this period. Chloris gayana KATR3 recorded highest average NDF (72.9%), ADF (48.1%) and ADL (6.1%) content during this period. Xaraes, Marandu and Mulato II were the only cultivars able to achieve the highest metabolizable energy of 7.0 MJ/kg DM recorded for the grass cultivars throughout the harvest intervals. These results indicate that Brachiaria grasses can be a good source of forage for livestock and a boost to the forage resource base in the semi arid regions of Kenya. Further research is needed to quantify their productivity in both dry and wet periods and to assess the effect of feeding on animal production performance.
The naked mole rat (NMR) is a rodent that has gained importance as a biomedical research model for various conditions like hypoxic brain injury, cancer and nociception. This study was designed to investigate possible involvement of the noadrenergic receptor system in antinoception in the NMR, using the alpha-2 adrenergic receptor specific ligands clonidine (agonist) and yohimbine (antagonist) in the formalin test. Formalin test followed 30 min after intraperitoneal administration of ligands or control. A total of 96 naked mole rats were used. A significant reduction in nociceptive behaviours was demonstrated after administration of clonidine in the doses 1,3,10 and 30 μg/kg (n = 8 per group). Doses of clonidine above 30 μg/kg caused loss of motor and proprietion skills exhibited by prostration and failure to turn over when placed on their backs. The antinociception by 3 μg/kg clonidine was reversed by administration of 30 μg/kg of yohimbine. The present study demonstrates that the noradrenergic receptor system is present and involved in formalin test-related antinociceptive mechanisms in the NMR, similar to other mammals. Given the increasing importance of the NMR as a model for pain and nociception, the species may prove useful as an animal model for noradrenergic mechanisms in pain modulation.
Objective The naked mole rat (NMR) (Heterocephalus glaber) is increasingly considered an important biomedical research model for various conditions like hypoxic brain injury, cancer and nociception. This study was designed to investigate the effects of clonidine and yohimbine, an alpha-2 (α2) adrenoceptor agonist and antagonist respectively in the tail flick and hot plate tests. Results A significant difference in tail flick latency was noted between saline control and 30 µg/kg clonidine, which was reduced after administration of 30 µg/kg yohimbine. A significant difference in hot plate latency was also noted between saline control and 30 µg/kg clodinine during the periods 30, 45, 60, 75 and 90 min after administration, and between saline control and 10 µg/kg clonidine during 30 min after administration. The hot plate latency by 30 µg/kg clonidine was also reduced by 30 µg/kg yohimbine during 30 min after administration. Since the tail-flick and hot plate tests mediate the effects at spinal and supraspinal levels respectively, the present study indicates the presence and involvement of noradrenergic receptors in thermal antinociception at spinal and supraspinal levels of the NMR, similar to what has been found in other mammals.
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