In vitro gas studies require the use of cannulated animals; however, the high cost of the surgery limits its application. Therefore, the objective of the study was to evaluate the effect of the ruminal fluid extraction technique by oro-ruminal probe affects the results derived from the in vitro gas production technique (GPT). Elaeis guineensis meal was used as a substrate. Ruminal fluid was obtained in two ways: cannulated animals and oro-ruminal probe. Dry matter degradation was estimated (DMD), parameters of fermentation kinetics and fractional volume by the GPT. The comparison of means was carried out using the Tukey procedure (p<0,05). The results indicated the lack of significant statistical differences (p>0,05). It was concluded that the ruminal fluid extraction technique did not modify the DGRMS, the parameters and fermentation fractions of E. guineensis.
<pre> </pre><pre>The objective was determine the degradation and kinetics of <em>in vitro</em> fermentation of excreta of excreta produced and marketed for the feeding of ruminants in the state of Chiapas, Mexico. Twelve samplings were made once the reproductive cycle of two chicken and pig producing farms was completed. A bromatological analysis was performed on the excreta samples (dry matter, organic matter, ash, crude protein, neutral detergent fiber and acid detergent fiber), <em>in vitro</em> gas production (maximum volume, rate, lag phase, fractions of fast, medium and slow fermentation), hemicellulose and copper content. A completely randomized design was used, and the comparison of means was performed using the Tukey procedure (p<0,05). The poultry manure has greater (p<0,05) content of organic matter than swine excreta, which is reflected in a higher (p<0,05) maximum volume (MV) of fermentation gas. The swine excreta it has a lower crude protein content and higher copper content compared to the poultry manure (p<0,05), as well as higher concentration of acid detergent fiber (ADF), thus, the poultry manure present better parameters (MV, rate and lag phase) and fractions of fermentation compared to the swine excreta. It is concluded that the poultry manure they have a better chemical composition tan swine excreta, possibly the latter caused by the high copper content of this, suggesting its limited use in animal feed.</pre>
En la crianza artificial de becerras es necesario reemplazar la leche para disminuir los costos de alimentación. Por ello, el objetivo del trabajo fue evaluar el uso de lactosuero fresco y enriquecido como sustituto de la leche entera en el sistema de crianza artificial de becerras Jersey. Se utilizaron 24 becerras de 31 ± 2,8 kg de peso, repartidas en tres tratamientos: T1) leche entera + concentrado + pasto (Cynodon nlemfuensis), T2) lactosuero fresco enriquecido + concentrado + pasto y T3) lactosuero fresco + concentrado + pasto. Se evaluó ganancia diaria de peso, consumo de concentrado, parámetros de fermentación ruminal y degradación in vitro del pasto. Se utilizó un diseño completamente al azar. La ganancia de peso fue similar para T1 y T2 (0,840 kg y 0,780 kg; p < 0,05), mientras que el consumo de concentrado fue similar para todos los tratamientos. El volumen máximo (VM: T1: 174,36 mL/g de MS, T2: 196,30 mL/g de MS y T3: 200,46 mL/g de MS; p > 0,05) y fase lag (L: T1: 1,36 h; T2: 1,22 h y T3: 0,68 h; p > 0,05) del pasto fue similar entre los tratamientos evaluados. A los 30 días, la mayor degradación se encontró en T1; sin embargo, a los 90 días no se encontraron diferencias. Se concluye que el uso del lactosuero fresco enriquecido es viable para reemplazar la leche en la crianza artificial de becerras Jersey.
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