This study evaluated the effect of Matricaria chamomilla and vaccination frequency on cattle immunization against rabies. Four groups (n = 15 /group) were treated with or without Matricaria chamomilla CH12 and vaccinated with one or two doses of rabies vaccine (30 day interval). No effect of chamomile was found on cattle immunization against rabies; however, antibody titers were protective in cattle vaccinated twice, while 93.3% of cattle vaccinated only once had titers under 0.5 UI/ml after 60 days. In conclusion, the use of chamomile did not alter the humoral immune response in cattle, and two vaccine doses are suggested for achieving protective antibody titers.
Four rabies antigen batches were produced from virus suspensions resulting from BHK21 cells adhered to microcarriers (Cytodex 1), inoculated and cultured in a bioreactor. In parallel the methodology of production of rabies virus through cultures of BHK21 cells in monolayers in bottles was used. The results obtained showed that infecting titles were 106.69 DL50/mL and 107.28 DL50/mL for suspensions cultured in bottles and in the bioreactor, respectively. The viral suspension volumes collected were on average 11,900 per batch from the bioreactor and 800mL per bottle. Ten horses were immunized with the antigen produced in the bioreactor. The means of antirabies antibody titers found were 240 and 212 IU/mL after the initial and the first booster doses, respectively. Rabies antigen with satisfactory infecting titers can be obtained on a large scale by culturing in a bioreactor inoculated BHK21 cells adhered to microcarriers.
Foram produzidos quatro lotes de antígeno rábico a partir de suspensões de vírus resultantes de células BHK21 infectadas, aderidas a microcarregadores do tipo Cytodex 1 e cultivadas em biorreator. Em paralelo foi utilizada a metodologia de produção de vírus rábico com células BHK21 em monocamadas, contidas em garrafas de 350cm2. Os resultados encontrados demonstraram que os títulos infectantes foram de 106.69 DL50/mL para as suspensões virais obtidas em garrafas e 107.28 DL50/mL para as do biorreator. Os volumes das suspensões virais colhidas foram, em média de 11.900mL por lote do biorreator e 800mL por garrafa . Com o antígeno produzido no biorreator foram imunizados 10 cavalos. As médias dos títulos de anticorpos anti-rábicos encontrados no soros destes animais foram de 240 e 212 UI/mL, respectivamente após a base e o primeiro reforço. Através da infecção de células BHK21 aderidas a microcarregadores e cultivadas em biorreator, pode-se obter antígeno rábico em larga escala e com títulos infectantes satisfatórios
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.