The enormous potential in diversity of the marine life is still not fully exploited due to the difficulty in culturing many of the microorganisms under laboratory conditions. In this mini-review we underlined the importance of using an omics technique, such as metagenomics, to access the uncultured majority of microbial communities. We report examples of several hydrolytic enzymes and natural products isolated by functional sequenced-based and function-screening strategies assisted by new high-throughput DNA sequencing technology and recent bioinformatics tools. This article ends with an overview of the potential future perspectives of the metagenomics in bioprospecting novel biocatalysts and bioactive compounds from marine sources.

The results of this study may be helpful for diagnosis and interpretation of data in patients with findings suggestive of a cholesterol biosynthesis defect.

No abstract

The results show that this enzymatic method is suitable to analyze cholesterol in DBS and it could be automated and used for population screening of total blood cholesterol.