In vitro experiments were performed with Gymnodinium catenatum Graham strains isolated from three locations in the Gulf of California to determine the variability in toxicity and toxin profiles. Strains were cultivated in GSe at 201 C AE 11 C, 150 lmol photons . m À 2 . s À 1 (12:12 light:dark cycle), and harvested during different growth phases. Growth rates were higher than in previous studies, varying between 0.70 and 0.82 day À 1 . The highest cell yields were reached at 16 and 19 days, with maximum densities between 1090 and 3393 cells . mL À 1 . Bahía de La Paz (BAPAZ) and Bahía de Mazatlán (BAMAZ) were the most toxic (101 pg STXeq . cell À 1 ), whereas strains from Bahía Concepción (BACO) were the least toxic (13 pg STXeq . cell À 1 ). A strain isolated from cyst germination was one of the least toxic strains. No significant changes in toxin content with culture age were observed (0.2 and 0.6 pmol paralytic shellfish poisoning . cell À 1 ). All strains contained neosaxitoxin (NEOSTX), decarbamoyl-saxitoxin (dcSTX), decarbamoyl-gonyautoxin-2,-3, (dcGTX2-3), N-sulfocarbamoylsaxitoxin (B1), N-sulfo-carbamoylneosaxitoxin (B2), and N-sulfo-carbamoylgonyautoxin-2,-3 (C1-2). Bahía Concepción strains had the highest content of C1; BAPAZ and BAMAZ strains had a higher percentage of NEOSTX. Differences in toxin composition with culture age were observed only in BAMAZ and BAPAZ strains. Cultures with a higher percentage of long chains had more NEOSTX, while those with a higher proportion of short chains had a lower content of NE-OSTX. Gulf of California strains are characterized by a high proportion of NEOSTX, and seem to have evolved particular physiological responses to their environment that are reflected in the toxin profile, suggesting different populations.
Harmful Algal Blooms (HABs) are becoming an increasing problem to human health and environment (including effects on natural and cultured resources, tourism and ecosystems) all over the world. In Mexico a number of human fatalities and important economic losses have occurred in the last 30 years because of these events. There are about 70 species of planktonic and non-planktonic microalgae considered harmful in Mexican coasts. The most important toxin-producing species are the dinoflagellates Gymnodinium catenatum and Pyrodinium bahamense var. compressum, in the Mexican Pacific, and Karenia brevis in the Gulf of Mexico, and consequently the poisonings documented in Mexico are Paralytic Shellfish Poisoning (PSP) and Neurotoxic Shellfish Poisoning (NSP). Although there is evidence that Amnesic Shellfish Poisoning (ASP), Diarrhetic Shellfish Poisoning (DSP) and Ciguatera Fish Poisoning (CFP) also occur in Mexico, these problems are reported less frequently. The type of phytoplankton and epiphytic microalgae, their toxins and harmful effects as well as current methodology used to study these phenomena are presented in this paper. As an experienced group of workers, we include descriptions of monitoring and mitigation programs, our proposals for collaborative projects and perspectives on future research.
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