Extracts of plants presents a large chemical diversity and usually require extensive time for the isolation and characterization of the compounds. Analysis of complex samples by electrospray ionization source coupled to a mass spectrometry (ESI-MS) and tandem mass spectrometry (ESI-MS/MS) has proven to be useful tools for initial screening of these samples. Therefore, it allows identification of known molecules without isolation or use of other auxiliary analytical techniques. In this work, dried extracts of three Byrsonima species were dissolved in methanol, analyzed through direct injection by ESI-MS and each reason mass-to-charge (m/z) of interest was selected to ESI-MS/MS analysis. ESI-MS fingerprint showed a distinction among the chemical constituents in each of the three Byrsonima species and ESI-MS/MS data was used as a tool to help identify the main components of B. coccolobifolia, B. verbascifolia and B. intermedia extracts. More specifically, phenolic acids, proanthocyanidins and flavonoids were detected in the three Byrsonima species studied.
Two new compounds, caryopristimerin and 2α,3α,22β-trihydroxy-21-oxo-29-nor-friedelan-24-oic acid, were isolated from the hexane/ethyl ether extract of Salacia crassifolia roots. Caryopristimerin represents the first example of a homo Diels-Alder adduct of a sesquiterpene and a triterpene, and the new 29-nor-friedelane displays a highly oxygenated A ring with a carboxylic group at the unusual C-5 position. The new compounds were elucidated by infrared (IR), highresolution-atmospheric pressure chemical ionization-mass spectrometry (HR-APCI-MS), 1D/2D nuclear magnetic resonance (NMR) and single crystal X-ray diffraction analysis. Additionally, the known compounds 3-oxo-29-hydroxyfriedelane, pristimerin, tingenone and netzahualcoyonol are herein reported for the first time as constituents of S. crassifolia. Their structures were established by spectroscopic analysis.
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