Medicinal plants are known for their diverse use in the traditional medicine of the Himalayan region of Pakistan. The present study is designed to investigate the anticancer and antimicrobial activities of Prunus cornuta and Quercus semicarpifolia. The anticancer activity was performed using cancerous human cell lines (HepG2, Caco-2, A549, MDA-MB-231, and NCI-H1437 carcinoma cells), while the antimicrobial activity was conducted with the agar-well diffusion method. Furthermore, toxicity studies were performed on alveolar and renal primary epithelial cells. Initially, different extracts were prepared by maceration techniques using n-hexane, chloroform, ethyl acetate, butanol, and methanol. The preliminary phytochemical screening showed the presence of secondary metabolites such as alkaloids, tannins, saponins, flavonoids, glycosides, and quinones. The chloroform extract of P. cornuta (PCC) exhibited significant inhibitory activity against Acinetobacter baumannii (16 mm) and Salmonella enterica (14.5 mm). The A. baumannii and S. enterica strains appeared highly susceptible to n-hexane extract of P. cornuta (PCN) with an antibacterial effect of 15 mm and 15.5 mm, respectively. The results also showed that the methanolic extracts of Quercus semecarpifolia (QSM) exhibited considerable antibacterial inhibitory activity in A. baumannii (18 mm), Escherichia coli (15 mm). The QSN and QSE extracts also showed good inhibition in A. baumannii with a 16 mm zone of inhibition. The Rhizopus oryzae strain has shown remarkable mycelial inhibition by PCM and QSN with 16 mm and 21 mm inhibition, respectively. Furthermore, the extracts of P. cornuta and Q. semicarpifolia exhibited prominent growth inhibition of breast (MDA-MB-231) and lung (A549) carcinoma cells with 19–30% and 22–39% cell viabilities, respectively. The gut cell line survival was also significantly inhibited by Q. semicarpifolia (24–34%). The findings of this study provide valuable information for the future development of new antibacterial and anticancer medicinal agents from P. cornuta and Q. semicarpifolia extracts.
Zinc oxide nanoparticles (ZnONPs) are the top candidate in the field of biological applications because of their high surface area and excellent catalytic activities. In the present study, the cyanobacteria-mediated biosynthesis of zinc oxide NPs using Nostoc sp. extract as a stabilizing, chelating, and reducing agent is reported. ZnONPs were biologically synthesized using an eco-friendly and simple technique with a minimal reaction time and calcination temperature. Various methods, including X-ray diffraction (XRD), ultraviolet spectroscopy (UV), Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) were used to characterize the biosynthesized zinc oxide NPs. XRD analysis depicted the crystalline form of zinc oxide NPs, and the Scherrer equation determined a mean crystalline size of ~28.21 nm. The SEM results reveal the spherical shape of the biosynthesized nanoparticles. Various functional groups were involved in the capping and stabilization of the zinc oxide NPs, which were confirmed by FTIR analysis. The zinc oxide NPs showed strong UV-vis absorption at 340 nm. Multiple in vitro biological applications showed significant therapeutic potential for zinc oxide NPs. Potential antimicrobial assays were reported for zinc oxide NPs via the disc-diffusion method and food poisoning method, respectively. All other activities mentioned below are described with the concentration and IC50 values. Biocompatibility with human erythrocytes and macrophages (IC50: 433 µg/mL, IC50 > 323 µg/mL) and cytotoxic properties using brine shrimps (IC50: 11.15 µg/mL) and Leishmania tropics (Amastigotes IC50: 43.14 µg mL−1 and Promastigotes IC50: 14.02 µg mL−1) were determined. Enzyme inhibition assays (protein kinase and alpha amylase) were performed and showed strong potential. Free radical scavenging tests showed strong antioxidant capacities. These results indicate that zinc oxide NPs synthesized by Nostoc sp. have strong biological applications and are promising candidates for clinical development.
The current study aimed to investigate the viability and characteristics of Scenedesmus sp. as an adsorbent system to remove lead (Pb) and cadmium (Cd) through an in vitro exposure to a metal solution. In batch sorption experiments, the effects of pH, contact time, initial concentration of metal ions, and sorbent dosage on the adsorption process were trialed. The ideal biosorption conditions for each of the two metals were recorded. The biosorption process was quick, and the equilibrium times for the above-mentioned metals were recorded as 90 and 60 min, with removal percentages of 85% and 83%, respectively. The point zero charge of algal biomass was 4.5, which indicates a negative charge on the surface of the biosorbent. The model-based assessment of the biosorption process was revealed to have followed pseudo-second-order kinetics. The adsorption isotherms for lead and cadmium achieved a best fit with the Langmuir model, with monolayer biosorption capacities of 102 and 128 mg g−1, respectively. The desorption of both metals achieved more than 70% by using HCl. The FT-IR revealed the presence of hydroxyl and amine groups on the surface of the adsorbent that are involved in the biosorption process, and morphological changes were assessed by SEM. Hence, Scenedesmus sp. from a Himalayan provenance showed considerable promise as an alternate sorbent for the treatment of heavy-metal-contaminated wastewater.
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