Inanimate hospital environment can serve as reservoirs of potential pathogen and is important in chain of infection. Health care-associated infections (HAI) remain a major cause of patient morbidity and mortality. In this study we comparative spread of bacteria in high and low contact surfaces in one tertiary care hospital in Iranian. Materials and Materials The present study was performed during a 30 month period a total study 194 environmental Bacteria. Environmental samples collected from low and high contact surface with swab in TSB. Identification of bacteria, were performed with microbiological methods. Results According to result, coagulase negative Staphylococcus species, consist the more bacteria isolated from high and low contact hospital surfaces. Conclusion Some microbes can survive on surfaces for long times. These environmental surfaces can be involved in the transfer of microorganisms to patients and subsequently in the development of hospital-associated infections.
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