A rapid microwave‐hydrothermal method has been developed to prepare monodisperse colloidal carbon nanospheres from glucose solution, and gold nanoparticles (AuNPs) are successfully assembled on the surface of the colloidal carbon nanospheres by a self‐assembly approach. The resulting AuNP/colloidal carbon nanosphere hybrid material (AuNP/C) has been characterized and is expected to offer a promising template for biomolecule immobilization and biosensor fabrication because of its satisfactory chemical stability and the good biocompatibility of AuNPs. Herein, as an example, it is demonstrated that the as‐prepared AuNP/C hybrid material can be conjugated with horseradish peroxidase‐labeled antibody (HRP‐Ab2) to fabricate HRP‐Ab2‐AuNP/C bioconjugates, which can then be used as a label for the sensitive detection of protein. The amperometric immunosensor fabricated on a carbon nanotube‐modified glass carbon electrode was very effective for antibody immobilization. The approach provided a linear response range between 0.01 and 250 ng mL−1 with a detection limit of 5.6 pg mL−1. The developed assay method was versatile, offered enhanced performances, and could be easily extended to other protein detection as well as DNA analysis.
A combined hydrothermal/hydrogen reduction method has been developed for the mass production of helical carbon nanotubes (HCNTs) by the pyrolysis of acetylene at 475 °C in the presence of Fe(3)O(4) nanoparticles. The synthesized HCNTs have been characterized by high-resolution transmission electron microscopy, scanning electron microscopy, X-ray diffraction analysis, vibrating sample magnetometry, and contact-angle measurements. The as-prepared helical-structured carbon nanotubes have a large specific surface area and high peroxidase-like activity. Catalysis was found to follow Michaelis-Menten kinetics and the HCNTs showed strong affinity for both H(2)O(2) and 3,3',5,5',-tetramethylbenzidine (TMB). Based on the high activity, the HCNTs were firstly used to develop a biocatalyst and amperometric sensor. At pH 7.0, the constructed amperometric sensor showed a linear range for the detection of H(2)O(2) from 0.5 to 115 μM with a correlation coefficient of 0.999 without the need for an electron-transfer mediator. Because of their low cost and high stability, these novel metallic HCNTs represent a promising candidate as mimetic enzymes and may find a wide range of new applications, such as in biocatalysis, immunoassay, and environmental monitoring.
A versatile immunosensor using CdTe quantum dots as electrochemical and fluorescent labels has been developed for sensitive protein detection. This sandwich-type sensor is fabricated on an indium tin oxide chip covered with a well-ordered gold nanoparticle monolayer. Gel imaging systems were successfully introduced to develop a novel high-efficient optical immunoassay, which could perform simultaneous detection for the samples with a series of different concentrations of a target analyte. The linear range of this assay was between 0.1 and 500 ng/mL, and the assay sensitivity could be further increased to 0.005 ng/mL with the linear range from 0.005 to 100 ng/mL by stripping voltammetric analysis. The immunosensor showed good precision, high sensitivity, acceptable stability, and reproducibility and could be used for the detection of real sample with consistent results in comparison with those obtained by the ELISA method.
Recently, Pt-Y alloy has displayed an excellent electrocatalytic activity for oxygen reduction reaction (ORR), and is regarded as a promising cathode catalyst for fuel cells. However, the bulk production of nanoscaled Pt-Y alloy with outstanding catalytic performance remains a great challenge. Here, we address the challenge through a simple dealloying method to synthesize nanoporous Pt-Y alloy (NP-PtY) with a typical ligament size of ~5 nm. By combining the intrinsic superior electrocatalytic activity of Pt-Y alloy with the special nanoporous structure, the NP-PtY bimetallic catalyst presents higher activity for ORR and ethanol oxidation reaction, and better electrocatalytic stability than the commercial Pt/C catalyst and nanoporous Pt alloy. The as-made NP-PtY holds great application potential as a promising electrocatalyst in proton exchange membrane fuel cells due to the advantages of facile preparation and excellent catalytic performance.
The inhibitor effect of the environmentally friendly corrosion inhibitor polyaspartic acid (PASP) on the corrosion of carbon steel in 0.5 M H 2 SO 4 was investigated by weight loss, potentiodynamic polarization, electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). Polarization curve results clearly reveal the fact that PASP is a good anode-type inhibitor. EIS results confirm its corrosion inhibition ability. The inhibition efficiency increases with increasing PASP concentration, and the maximum inhibition efficiency was 80.33% at 10 8C. SEM reveals that a protective film forms on the surface of the inhibited sample. The adsorption of this inhibitor is found to follow the Freundlich adsorption isotherm. A mechanism is proposed to explain the inhibitory action of the corrosion inhibitor.
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