HCV core induces loss of polarity and down-regulates SHIP2 and Dlg1 expression. SHIP2 and PtdIns(3,4)P2 are localized at the basolateral membrane of polarized cells. SHIP2 siRNA and its catalytically inactive mutant disrupt epithelial polarity, and SHIP2 rescues core-induced loss of polarity through RhoA activation.
We have examined the Acylated Ghrelin (AG)/Gi pathway in different human osteoblastic cell lines. We have found that: 1) AG induces differentiation/mineralization only in mature osteoblasts; 2) the expression of GHS-R1a increases up to the mature cell stage, 3) the action is mediated via the GHS-R/Gi/cAMP pathway only in mature osteoblasts, and 4) osteoblastic cells from adolescent idiopathic scoliosis (AIS) are resistant to the AG/Gi/cAMP pathway. Altogether, these results suggested that AG uses the GHS-R1a/Gi/cAMP pathway to induce differentiation in mature osteoblasts only. This pathway is impaired in AIS osteoblasts. Understanding AG-specific pathways involved in normal and pathological osteoblasts may be useful for developing new treatments for pathologies such as AIS or osteoporosis.
Zusammenfassung: Es wird über den Nachweis von C. trachomatis-Infektionen mit einem Antigen-E/A bei Frauen und Männern verschiedenen Alters und mit häufig wechselnden Partnern berichtet. Alle Personen waren symptomfrei bzw. zeigten lediglich Erscheinungen einer Vaginitis bzw. Urethritis. Es wurde festgestellt, daß etwa ein Drittel der Untersuchten (32%) ein positives Ergebnis aufwiesen, welches auf eine bis dahin unbekannte Infektion mit C. trachomatis hindeutete. Der Antigen-EIA ist geeignet, die meisten Infektionen zu erkennen und bei behandelten Patienten den Erfolg der Therapie zu kontrollieren. Schlüsselwörter: Chlamydia trachomatis -genitale Kontaktinfektionen -Chlamydia-Antigennachweis Summary: Investigations on the detection of C. trachomatis antigens by an El A in promiscuitive women and men are presented. All persons were either without clinical Symptoms of infection or showed only a vaginitis pr an urethritis. It is shown that 32% of investigated patients had a positive test result demonstrating an at that time unknown C. trachomatis infection. The antigen EIA is suited to control the effectiveness of specific therapy.
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