The initial steps of the human immunodeficiency virus 1 (HIV-1) lifecycle are regulated by cellular RNA-binding proteins (RBPs). To understand the scope of these early host-virus interactions, we developed in virion RNA interactome capture (ivRIC), which allowed the comprehensive and systematic profiling of the proteins that interact with the HIV-1 genomic (g)RNA inside viral particles. ivRIC identified 104 cellular RBPs within the encapsidated HIV-1 ribonucleoprotein, many of which are typically found in the cellular nucleus. Notably, these nuclear RBPs interact with the HIV-1 RBP Rev, suggesting that they associate with HIV-1 gRNA during its nuclear life. Functional assays show that ivRBPs are important for HIV-1, including PURA and PURB, which control viral gene expression and infectivity through interaction with critical sequences in the gRNA. Our characterisation of the composition of the encapsidated ribonucleoprotein of HIV-1 uncovers new host-virus interactions that invokes new mechanisms for controlling HIV-1 infection.
The later 1550s in Sprain are remembered as the time of the discovery of ‘Lutheran’ groups in Valladolid and Seville and the ensuing reaction of the authorities: theautos de fe, as the Fernando de Valdés'sIndex librorum prohibitorum, the edict ordering all Spaniards (with few exceptions) studying or teaching abroad to return to Spain within four months. ‘Heresy’ was to be eradicated at home and Spain was to be preserved from contagion from beyond her frontiers. There was, however, a further subordinate aim: to capture and bring back to Spain those Spaniards who for reasons of religion had taken refuge in Northern Europe. When a dozen Hieronymite monks from San Isidro at Seville fled their monastery and their country in the late summer of 1557, one finds the Inquisitor General, in November of that year, sending a list of their names to the king in the Netherlands. Further lists of wanted heretics continued to be forwarded there when Philip had returned to Spain. Cardinal Granvelle, in a letter to the king of 18 October 1561, refers to ‘aquella escriptura que V. M. me embió venida de los inquisidores de Sevilla’. At the start of that year he had similarly referred to ‘un escripto venido de los inquisidores de Sevilla’ which the king had recently sent. In April 1562 the Margrave of Antwerp was replying to inquiries from Granvelle concerning persons named in the trials of Julian Hernandez (caught when in Seville, distributing Protestant literature, in 1557) and Fray Domingo de Guzmán.
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