A complex of highly conserved proteins consisting of NusB, NusE, NusA, and NusG is required for robust expression of rRNA in Escherichia coli. This complex is proposed to prevent Rho-dependent transcription termination by a process known as “antitermination.” The mechanism of this antitermination in rRNA is poorly understood but requires association of NusB and NusE with a specific RNA sequence in rRNA known as BoxA. Here, we identify a novel member of the rRNA antitermination machinery: the inositol monophosphatase SuhB. We show that SuhB associates with elongating RNA polymerase (RNAP) at rRNA in a NusB-dependent manner. Although we show that SuhB is required for BoxA-mediated antitermination in a reporter system, our data indicate that the major function of the NusB/E/A/G/SuhB complex is not to prevent Rho-dependent termination of rRNA but rather to promote correct rRNA maturation. This occurs through formation of a SuhB-mediated loop between NusB/E/BoxA and RNAP/NusA/G. Thus, we have reassigned the function of these proteins at rRNA and identified another key player in this complex.

Predictable latency on flash storage is a long-pursuit goal, yet, unpredictability stays due to the unavoidable disturbance from many well-known SSD internal activities. To combat this issue, the recent NVMe IO Determinism (IOD) interface advocates host-level controls to SSD internal management tasks. While promising, challenges remain on how to exploit it for truly predictable performance. We present IODA , an I/O deterministic flash array design built on top of small but powerful extensions to the IOD interface for easy deployment. IODA exploits data redundancy in the context of IOD for a strong latency predictability contract. In IODA , SSDs are expected to quickly fail an I/O on purpose to allow predictable I/Os through proactive data reconstruction. In the case of concurrent internal operations, IODA introduces busy remaining time exposure and predictable-latency-window formulation to guarantee predictable data reconstructions. Overall, IODA only adds 5 new fields to the NVMe interface and a small modification in the flash firmware, while keeping most of the complexity in the host OS. Our evaluation shows that IODA improves the 95–99.99þ latencies by up to 75 ×. IODA is also the nearest to the ideal, no disturbance case compared to 7 state-of-the-art preemption, suspension, GC coordination, partitioning, tiny-tail flash controller, prediction, and proactive approaches.

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