Here we demonstrate that SPIM-FCS/FCCS possesses the sensitivity to detect and quantify protein-protein interactions in live cells by characterizing the interaction of the subunits of heterodimeric transcription factors, c-Fos/c-Jun and IQGAP/cdc42. The protein-protein interaction clearly shows up in the cross-correlation amplitude. Analysis of the spatial distribution of diffusion coefficients of the fluorescent proteins and of their cross-correlation amplitude shows that formation of the heterodimer is correlated with regions of decreased mobility, probably related to DNA binding.
Picosecond lasers sources operating over an order of magnitude in repetition rates and pulse energies at selected emission wavelengths from the UV to IR range are presented for time-correlated single-photon counting in demanding imaging applications.
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