This paper deals with the analysis of a Surface Plasmon Resonance (SPR) based D-Shape Photonic Crystal Fiber (PCF) Biosensor for the detection of multi-molecules present in a single analyte or simultaneous detection of more than one samples of an analyte. A spatially distributed bi-metallic layer of plasmonic metals (Silver and Gold) are deposited on the outer flat surface of a D-shaped PCF. Two metals generate two resonance peaks corresponding to a specific sample of an analyte. The analytes are floated on to the flat surface of D-shaped PCF. The mode analysis of proposed sensor is done using Finite Element Method (FEM) with Perfectly Matched Layer (PML). In this study, it can be seen that spatially distributed bimetallic layers can detect more than one sample of analyte simultaneously. The different dimensions of spatially distributed bimetallic SPR based D-Shape PCF sensor are also optimized with respect to centre-hole diameter, first air hole ring diameter, metal layer thickness and the depth of D-shape. It is also observed that the two SPR wavelengths can be controlled slightly by varying these parameters. It has been seen that for some optimal set of dimensions with the same sensitivity, the confinement loss of the plasmonic peaks is more. This property may be useful in the applications, where one can sense more than two molecules or more than two samples for the single analyte.
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