The aims of this study were to investigate: i) if a specific strain(s) became predominant in the stomach of mice infected with multiple strains of freshly isolated H. pylori; ii) if a time dependent selection of strains occurs; iii) to compare the degree of gastric inflammation in C57BL/6, CBA/Ca, BALB/cA and NMRI mice; and iv) to follow the serological response to the infection. Six weeks old mice were inoculated orally 3 times at 2-day intervals with 9 freshly isolated H. pylori strains. Five animals of each mouse strain were sacrificed at 2, 4, 10 and 16 weeks post-inoculation. Stomach smears were cultured on GAB-Camp agar at 37°C for 5 -10 days under microaerophilic conditions. Seven to 10 single H. pylori colonies from each animal were subcultured and identified by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. Histopathology of the gastric mucosa and serum antibody response were also analysed for each animal.Most mice were found to be infected by 1 strain (119/95). In only a few animals were multiple strains recovered. Strain 119/95 constituted 90.5% of the 577 mouse-passaged isolates and when tested it expressed both vacuolating cytotoxin antigen (VacA) and cytotoxin associated antigen (CagA). The inflammation scores of murine stomachs and duodenum samples of infected animals were significantly higher than in non-infected control animals for all 4 mouse strains. No significant differences in inflammation score were observed for the mouse strains 16 weeks post-infection. The immune response was also higher in infected versus non-infected animals for all mouse strains. The specific serum anti H. pylori immune response was highest for the NMRI mice followed by BALB/cA, C57BL/6, and CBA/Ca mice.
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