Microlicia deflexasp. nov. and M. johnwurdackianasp. nov. are described, illustrated and an updated identification key for the species of Microlicia from Clube Caça e Pesca Itororó de Uberlândia is provided. Microlicia deflexa occurs in Minas Gerais and Goiás States and is characterised by its linear-lanceolate and deflexed sepal on flower and immature fruit, long pedicel and indumentum of glandular trichomes, mixed with spherical, golden glands. Microlicia johnwurdackiana is endemic to Uberlândia and characterised by having indumentum of setose trichomes and spherical, golden glands, magenta petal with greenish abaxial surface at the apex and dimorphic stamens with bicolorous and tetrasporangiate anthers.