-(Identification of flavonoids in Hypericum cordatum (Vell.) N. Robson (Clusiaceae)). The aim of this work was the fractionation of methanolic extracts from stems and leaves of Hypericum cordatum and the identification of flavonoids. The fractionation of methanolic extracts was done using filtration columm in Sephadex LH 20 resin, thin layer and high performance liquid chromatography techniques. Several flavonoids were found in the methanolic extracts, specially flavonols and flavones. Four flavonoids were identified: quercetin, quercitrin, rutin and kaempferol. The identification of these compounds was done by the analyses of the ultraviolet light spectrum obtained in HPLC-UV/DAD and by co-chromatography with standard solutions.Key words -"Cerrado", flavonoids, medicinal plant, phenolic compounds, Saint John's worth RESUMO -(Identificação de flavonóides em Hypericum cordatum (Vell.) N. Robson (Clusiaceae)). Este trabalho teve como objetivo realizar o fracionamento de extratos metanólicos de caules e folhas de Hypericum cordatum e a identificação de flavonóides. O fracionamento dos extratos foi feito por cromatografia de filtração em resina de Sephadex LH 20, em camada delgada e cromatografia líquida de alta eficiência. Os extratos metanólicos mostraram a presença de vários flavonóides, em especial flavonóis e flavonas. Foram identificados quatro flavonóides: quercetina, quercitrina, rutina e canferol. Os compostos foram identificados pela análise de seus espectros na luz ultravioleta obtidos em CLAE-UV/DAD e por co-cromatografia realizada com os padrões.Palavras-chave -Cerrado, compostos fenólicos, erva de São João, flavonóides, planta medicinal.
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