Yuclei of the spor;lngiospores of seven strains ant1 thc gro\\.i~~g liyphae of fi1.e strains of Pl~ycomyces b l c~k e~l e~~(~~~~~. s have been esaminecl ill fixecl ancl stained prepnr;ltions. Onc strain was found to havc ~~~~~~s u a l l y large nuclei aticl has been studied in detail. 'I'hc nuclei in the sporar~giospores consist of Feulgen-positi\.e granules and short filaments in a diffuse matrix. I n the ordinar). strains the nuclei occur in a narro\lT range of sizes with their largest cliarncters around 2 ~nicrons. I n thc spores of the unusual culture the nuclei are of several sizes ancl nlan! have cliameters around 4 ancl 5 microns. 5imilar dilrerences are fo~und between the nuclei in gro\ving rn).celia. 'l'he nuclei in h).ph;~e resemble those of Mlrror Icicmc~lis. 'They consist oi a shell of tightly packed granules ancl lilaments of chromatin curving around a relati\~ely large n~~clcolus. 'The nuclei divide by elongation follo\\lecl by col~strictioli. The ~~~~c l e o l u s divides a t the same tinie ancl in the same \\;a). ancl half of it passes to each sister nllcleus. 'l'hc possibility is discussecl that the strain \\~ith the large nuclei may ha1.e hat1 its origin in a spore formed 11). a ,germinating zygote arlcl colltainillg both haploid and diploid nuclci. 'l'he nucle~ of the unl~su;il ci~lture, lirst esaminecl 3 years ago, hn\;e preser\,ecl their large size through man! c!.cles of \.egetative propaga tion. LII;lnuscript receivccl March 5 , 1957. Contributioll from the Uepart~nent of Bacteriology, University of Wester11 Ontario, Facult\. of Meclicine. Lonclon. Canada. This research was S U D D V~~~~ bv a grant from the
1974. Intracellular growth of Bdellovibrio bacteriovorirs 6-5-S in heat-killed Spirillur?~ serpetis VHL. Can. J. Microbiol. 20: 847-851. If cells of Spirillum serpens (strain VHL) are heat-killed at 56C for 90 min the cells retain their original morphology remarkably well. When actively motile cells of Bdellovibrio bacteriovorus (strain 6-5-S) are inoculated into a suspension of S . serpens heat-killed at this temperature, B. bacteriovorus attacks and penetrares the dead cells, and undergoes a cycle of growth and replication similar to that which occurs in living cells. Intracellular growth of B. bacteriovorus in nonviable cells has not previously been reported. Ross, E. J., C. F. ROBINOW et J. ROBINSON. 1974. Intracellular growth of Bdellovibrio bacteriovorus 6-5-S in heat-killed Spirillum serpens VHL. Can. J. Microbiol. 20: 847-851. Si des cellules de Spirilllon serpens (souche VHL) sont dttruites par chauffage a 56C pendant 90 min, c: s dernieres conservent tres bien leur morphologie originelle. Lorsque des cellules trts mobiles, de Bdellouibrio bacteriovorus (souche 6-56) sont inoculees dans des suspensions de S . serpeus detruites ii cette temperature, B. bacteriovonls attaque et penetre les cellules mortes et debute un cycle de croissance et de replication semblable a celui que I'on retrouve dans les cellules vivantes. C'est la premiere fois que Yon rapporte la croissance intracellulaire de B. bacteriovorus dans des cellules nonviables.[Traduit par le journal]
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