The foremost objective of this work is to prepare a novel electrochemical sensor-based screen-printed carbon electrode made of zinc oxide nanoparticles/molecularly imprinted polymer (SPCE–ZnONPs/MIP) and investigate its characteristics to detect sodium dodecyl sulfate (SDS).
The harmful impact of the heavy metal lead on human health has been known for years. However, materials that contain lead remain in the environment. Measuring the blood lead level (BLL) is the only way to officially evaluate the degree of exposure to lead. The so-called "safe value" of the BLL seems to unreliably represent the secure threshold for children. In general, lead's underlying toxicological mechanism remains unclear and needs to be elucidated. Therefore, we developed a novel genetically encoded fluorescence resonance energy transfer (FRET)-based lead biosensor, Met-lead, and applied it to transgenic Drosophila to perform further investigations. We combined Met-lead with the UAS-GAL4 system to the sensor protein specifically expressed within certain regions of fly brains. Using a suitable imaging platform, including a fast epifluorescent or confocal laser-scanning/two-photon microscope with high resolution, we recorded the changes in lead content inside fly brains ex vivo and in vivo and at different life stages. The blood-brain barrier was found to play an important role in the protection of neurons in the brain against damage due to the heavy metal lead, either through food or microinjection into the abdomen. Met-lead has the potential to be a powerful tool for the sensing of lead within living organisms by employing either a fast epi-FRET microscope or high-resolution brain imaging.
A rapid, simple, and sensitive voltammetric sensor has been fabricated to determine Rhodamine B (RhB), a textile coloring agent. Silver nanoparticles (AgNPs) were synthesized by the chemical reduction method of silver nitrate and sodium citrate. Graphene nanoplatelets (GPLs) and AgNPs were drop-casted on the surface of a working electrode of a screen-printed carbon electrode (SPCE), forming the SPCE-GPLs/AgNPs samples. Scanning electron microscopy−energy dispersive X-ray and cyclic voltammetry confirmed the altered surface of the SPCE. The square wave voltammetry was used for the electrochemical determination of RhB. The SPCE-GPLs/AgNPs demonstrated electrochemical responses to detect RhB with a linear range of 2−100 μM, and the limit of detection was 1.94 μM. The SPCE-GPLs/ AgNPs demonstrated a selective detection of RhB in the presence of common interfering compounds present in the food samples, including sucrose and monosodium glutamate. Furthermore, the sensor presented good reproducibility as well as repeatability in the detection of RhB. When the sensor was used to determine RhB in an actual food sample, similar results were shown as suggested by UV−vis spectroscopy analysis. Hence, the fabricated sensor can be applied for the detection of RhB in food samples.
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