We argue that BSS-REG may enable the development of novel BCI applications requiring high-density recordings, such as source-based neurofeedback and closed-loop neuromodulation.

Recent studies have highlighted the importance of an accurate individual head model for reliably using high-density electroencephalography (hdEEG) as a brain imaging technique. Correct identification of sensor positions is fundamental for accurately estimating neural activity from hdEEG recordings. We previously introduced a method of automated localization and labelling of hdEEG sensors using an infrared colour-enhanced 3D scanner. Here, we describe an extension of this method, the spatial positioning toolbox for head markers using 3D scans (SPOT3D), which integrates a graphical user interface (GUI). This enables the correction of imprecisions in EEG sensor positioning and the inclusion of additional head markers. The toolbox was validated using 3D scan data collected in four participants wearing a 256-channel hdEEG cap. We quantified the misalignment between the 3D scan and the head shape, and errors in EEG sensor locations. We assessed these parameters after using the automated approach and after manually adjusting its results by means of the GUI. The GUI overcomes the main limitations of the automated method, yielding enhanced precision and reliability of head marker positioning.

Objective. Electroencephalography (EEG) is a widely used technique to address research questions about brain functioning, from controlled laboratorial conditions to naturalistic environments. However, EEG data are affected by biological (e.g., ocular, myogenic) and non-biological (e.g., movement-related) artifacts, which -depending on their extent- may limit the interpretability of the study results. Blind source separation (BSS) approaches have demonstrated to be particularly promising for attenuation of artifacts in high-density EEG (hdEEG) data. Previous EEG artifact removal studies suggested that it may not be optimal to use the same BSS method for different kinds of artifacts. Approach. In this study, we developed a novel multi-step BSS approach to optimize the attenuation of ocular, movement-related and myogenic artifacts from hdEEG data. For validation purposes, we used hdEEG data collected in a group of healthy participants in standing, slow-walking and fast-walking conditions. During part of the experiment, a series of tone bursts were used to evoke auditory responses. We quantified event-related potentials (ERPs) using hdEEG signals collected during auditory stimulation, as well as event-related desynchronization (ERD) by contrasting hdEEG signals collected in walking and standing conditions, without auditory stimulation. We compared the results obtained in terms of auditory ERP and motor-related ERD using the proposed multi-step BSS approach, with respect to two classically used single-step BSS approaches. Main results. The use of our approach yielded the lowest residual noise in the hdEEG data, and permitted to retrieve stronger and more reliable modulations of neural activity than alternative solutions. Overall, our study confirmed that the performance of BSS-based artifact removal can be improved by using specific BSS methods and parameters for different kinds of artifacts. Significance. Our technological solution supports a wider use of hdEEG-based source imaging in movement and rehabilitation studies, and contribute to further development of mobile brain/body imaging applications.

High-density electroencephalography (hdEEG) has been successfully used for large-scale investigations of neural activity in the healthy and diseased human brain. Because of their high computational demand, analyses of source-projected hdEEG data are typically performed offline. Here, we present a real-time noninvasive electrophysiology toolbox, RT-NET, which has been specifically developed for online reconstruction of neural activity using hdEEG. RT-NET relies on the Lab Streaming Layer for acquiring raw data from a large number of EEG amplifiers and for streaming the processed data to external applications. RT-NET estimates a spatial filter for artifact removal and source activity reconstruction using a calibration dataset. This spatial filter is then applied to the hdEEG data as they are acquired, thereby ensuring low latencies and computation times. Overall, our analyses show that RT-NET can estimate real-time neural activity with performance comparable to offline analysis methods. It may therefore enable the development of novel brain–computer interface applications such as source-based neurofeedback.