The use of butyl-methyl-methacrylate embedding and the application of the silver methenamine (SM) method as a poststaining of the immunoperoxidase-DAB (IP) procedure led to the standardization of several useful methods for the visualization of tissue antigens at the light and electron microscope level. These procedures included: 1) Standardization of the actual methacrylate embedding; 2) The IP-SM method with and without periodic acid oxidation, which provided 100% intensification of the IP staining; 3) The IP-SM method made it possible to stain semithin sections (0.5 micron), and this in turn, permitted a) clear visualization under the light microscope of the intracellular distribution of antigens and, b) staining, in several adjacent sections, of roughly the same cytoplasmic region of the same cell with different primary antisera; 4) a double immunostaining whereby the first antigen in the sequence was revealed by the IP-SM method and the second by the IP procedure; 5) standardization of the IP and the IP-SM methods for post-embedding staining of ultrathin methacrylate sections. The combined application of methacrylate embedding and the IP-SM, and the use of an appropriate fixative, resulted in an ultrastructural immunocytochemical procedure characterized by a good immunoreactivity of the tissue sections, a strong and selective immunoreaction and a well preserved ultrastructure.
ResumenEn este estudio se caracteriza la fonética-fonología, gramática y léxico del mapudungún empleado en los collag (cantos) recopilados en el mar interior de Chiloé por Juan Elías Necul alrededor de 1887, pocos años antes de la extinción local del idioma. El trabajo se centra en el sistema de consonantes, el inventario y función de los sufijos verbales y el vocabulario empleado en los cantos. A partir de los resultados se concluye que esta variedad no parecía compartir los rasgos diferenciales del chesüngun hablado actualmente en la región de Los Lagos, que los sufijos de las formas no finitas se habían lexicalizado o habian adoptado otras funciones y que la mayor parte del vocabulario no difiere del vocabulario corriente en otras variedades de la lengua mapuche.Palabras clave: mapudungun, Chiloé, collag, veliche. Pillañ peuma rruaín: mapudungun in Chiloe Archipelago at late 19th century according to collag Abstract 1In the present study, phonetics-phonology, grammar and vocabulary of Mapuche language used in collag (songs) are characterized. Consonant system, inventory and function of verbal suffixes and vocabulary were analyzed. From results arise three main conclusions: Firstly, this variety apparently did not share the specific features of chesüngun dialect, currently spoken in Los Lagos Region. Secondly suffixes of non-finite forms became lexicalized or changed irregularly their functions. Lastly, most of the vocabulary present in collag was the same as other varieties of Mapuche language.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.