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Gene-based therapeutic strategies for cancer mainly packaging cell clone that produced a reasonable titer of include augmentation of immunotherapeutic and chemorecombinant virus and expressed high levels of IL-2 and tk therapeutic approaches. In this study we report the design transcripts. Although transduction efficiency was reduced and functional assay of a novel bicistronic Moloney-based in glioma cells as compared with murine NIH 3T3 cells, retroviral vector expressing human interleukin-2 (IL-2) and transgene expression was effectively achieved. Transherpesvirus thymidine kinase (tk) through a cap-dependent duced glioma cells were sensitive to ganciclovir and translation and an internal ribosome entry site (IRES)-regusecreted around 1000 U/ml IL-2 in the culture superlated translation, respectively. This construct has the natants. Simultaneous production of IL-2 and tk in vivo by potential for allowing combination of cytokine and suicide genetically treated tumor cells would hopefully potentiate gene therapy, especially in areas such as the brain, comthe effect of gangiclovir-induced metabolic suicide, posed of post-mitotic cells refractory to transduction by possibly by boosting the immune response associated type C retroviral vectors. Accordingly, human glioma cells with tumor debulking or by amplifying the bystander were used as targets for gene transfer after selecting a response.

A new restriction fragment length polymorphism (RFLP) analysis has been developed for hepatitis C virus (HCV) typing in the viral 5′ non-coding region and contiguous core region. These genomic sequences were chosen for the relative nucleotide homology among different genotypes and for the presence of polymorphic sites. By employing two endonucleases (AccI and MboI) and, in some instances, a third one (EcoRII), we can unambiguously and reproducibly distinguish between genotypes and subtypes 1a, 1b, 1c, 2a, 2c, 2b, 3a, 3b, 4a, 5a and 6a. The method was applied for diagnosing two Italian groups of HCV-infected individuals reflecting a randomly collected population and a group of intravenous drug users. The accuracy of this method has been validated by comparison with INNOLiPA and by sequencing. Our approach represents an improvement over previous RFLP methods, since typing is accurate and simpler.

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