Skin sensitizing agents that were present in a specific batch of alkyl ethoxy sulfate (AES) were identified as 1‐dodecene‐1,3‐sultone and 1‐tetradecene‐1,3‐sultone. Two other sensitizing agents that were present in the AES were tentatively identified as 2‐chloro‐1,3‐dodecanesultone and 2‐chloro‐1,3‐tetradecanesultone. A route of formation for these sensitizers has been postulated which is consistent with the specific conditions used for the manufacture of this batch of AES.
13C0,/12C0, ratios in the respired breath of rats fed carbon-13 labeled compounds have been continuously assayed using a mass spectrometer to monitor m/e 44 and 45 in real time. Rats dosed with sodium [l-l3C]acetate were restrained in a specially designed glass metabolism cage. A constant air flow through the cage was conbinuously monitored for respired carbon dioxide by a commercial analytical organic high resolution m a s spectrometer. The mass spectrometer was operated in the peak matchmg mode while alternately recording the ion intensities of m/e 44 and 45 at a cycle rate of 30 s. The appearance and decay in the breath of excess l3COZ was interpreted for the metabolism of sodium acetate. The minimum dose of label was found to be and limitations of the technique are discussed. In order to compare 13C02 results with an established radiochemical method the rats were also dosed with sodium [l-14C]acetate.The appearance of 14C0, iia the respired breath was followed by bubbling the breath through sodium hydroxide traps with quantitation of radioactivity by means of liquid scintillation counting. A comparison of the rates of respiration of 13C0, and 14C0, showed that the two methods produced comparable results in rats. This finding further substantiates the feasibility of using carbon-13 labeled compounds in metabolism studies.
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