97331 to the exit side of the gas trap and an air pump to the aeration frit, with valves on both the vacuum and air pump lines to adjust the pressure in the tanks to atmospheric pressure.

eNo B. R. Mere. 1975. Heavy metal and chlorinated hydrocarbon residues in California sea lions(Zalophus californianus californianus). J. Fish. Res. Board Can. 32: 2391-2397.Samples of various tissues and organs from healthy California sea lions (Zalophus californianus californianus) and sick animals (apparently with leptospirosis) collected along the central Oregon coast in 1970Oregon coast in , 1971Oregon coast in , and 1973 were analyzed for total mercury, methylmercury, cadmium, and chlorinated hydrocarbons. Maximum mercury concentrations of74-170 ppm occurred in sea lion liver, but only 1.6-3.7% of this was present as methylmercury. Cadmium was concentrated primarily in the kidney which containe d 7 .2-12.0 ppm of the metal. Chlorinated hydrocarbon residues in sea lion fat ranged between 253-475 ppm DDE, and 21.2 and 34.1 ppm pcB. Although mercury, cadmium, and chlorinated hydrocarbon residues in some of the sick sea lions were significantly higher than those present in healthy animals, it is not possible to relate these differences to the onset ofleptospirosis. BUHLER, D. R., R. R. Cr-en.vs, eNo B. R. Mere. 1975. Heavy metal and chlorinated hydrocarbon residues in California sea lions(Zalophus californianus californianus). J. Fish. Res. Board Can. 32: 2391-2397 . Nous avons dos6 le mercure total, le mercure-m6thyl, le cadmium et les hydrocarbures chlor6s dans des 6chantillons de divers tissus et organes d'otaries de Californie (Zalophus calilbrnianus californianus) en sant6 et d'otaries malades (apparamment atteintes de leptospirose) captur6es le long de la c6te de I'Or6gon central en 1970, 1971 et 1973. Les concentrations maximales de mercure de 74-170 ppm se rencontrent dans le foie, mais seulement 1.6-3.7Vo sous forme de mercure-m6thyl. Le cadmium est concentr6 surtout dans le rein qui contient 7.2-12.0ppm de ce m6tal. Les r6sidus d'hydrocarbures chlor6s dans des otaries grasses sont de 253415 ppm de DDE et de21.2-34.1ppm de BPC. Quoique les niveaux de mercure, de cadmium et de r6sidus d'hydrocarbures chlor6s chez quelques-unes des otaries malades soient plus 6lev6s que ceux des animaux sains, il nous a 6t6 impossible d'6tablir une relation entre ces diff6rences et le d€but de la leptospirose.

Florisil, alumina, and silicic acid were compared for the column chromatographic separation of lipids from chlorinated hydrocarbons. Alumina had the largest capacity per unit volume to retain lipids and oils (0.7 g beef lipid/ 25 g alumina) while eluting dieldrin. Florisil had a larger capacity than silicic acid. Deactivation and elution with a nonpolar solvent gave superior separation over previously used polar solvent elution from activated adsorbents. The effects of temperature, water, and lipid load on the elution of dieldrin were studied. A yellow dye, p-methoxyazobenzene, was found to have a slightly larger elution volume than dieldrin, and is useful as a visible reference material for the elution of chlorinated hydrocarbons.

Weanling rats have been raised for a period of 12 weeks on a ration containing 150 ppm. of DDT [ethane l,l,l-trichloro-2,2-bis(p-chlorophenyl)] and 22% alpha-protein as the sole source of nitrogen.

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