Ring glands dissected from homozygous l(3)ecd1ts wandering larvae and upshifted in vitro to the restrictive temperature, 29 degrees C, synthesize abnormally low quantities of ecdysteroid. Nevertheless, ecd1 ring glands retain the ability to respond at 29 degrees C to an extract prepared from wild-type larval neural tissues that presumably contain prothoracicotropic hormone (PTTH), although both basal and stimulated levels of synthesis are lower than those in wild-type ring glands. Extracts prepared from ecd1 neural tissue exhibit an unusually high level of PTTH activity. Mutant ring glands downshifted in vitro to the permissive temperature after removal from larvae maintained at 29 degrees C regain the ability to produce normal basal and stimulated ecdysteroid levels. Collectively, these experiments demonstrate that the ecd1 mutation disrupts the physiology of the ring gland at 29 degrees C autonomously and may also interfere with PTTH release.
The genetic organization of interval 62B3-4 to 62D3-4 on the Drosophila third chromosome was investigated. The region (designated DRE) includes four known loci: Roughened (R; 3-1.4), defined by a dominant mutation disrupting eye morphology; the nonvital locus Aprt, structural gene for adenine phosphoribosyltransferase; Dras3, a homolog of the vertebrate ras oncogene; and 1(3)ecdysoneless (1(3)ecd), a gene that has been implicated in the regulation of larval molting hormone (ecdysteroid) synthesis. Overlapping chromosomal deletions of the region were generated by gamma-ray-induced reversion of the R mutation. Recessive lethal mutations were isolated based upon failure to complement the recessive lethality of Df(3L)RR2, a deletion of the DRE region that removes 16-18 polytene chromosome bands. A total of 117 mutations were isolated following ethyl methanesulfonate and gamma-ray mutagenesis. These and two additional define 13 lethal complementation groups. Mutations at two loci were recovered at disproportionately high rates. One of these loci is preferentially sensitive to radiation-induced mutational alterations. Additionally, an unusually low recovery rate for cytologically detectable rearrangement breakpoints within the gamma-ray-sensitive locus suggests that an interval of the DRE region closely linked to the R locus may be dominantly sensitive to position effects. Lethal phase analysis of mutant hemizygotes indicates that a high proportion of DRE-region loci (11 of 13) are necessary for larval development. Mutations in five loci cause predominantly first-instar larval lethality, while mutations in four other loci cause predominantly second-instar lethality. Mutations in two loci cause late-larval lethality associated with abnormal imaginal disc development. A temperature-sensitive allele of one newly identified complementation group blocks ecdysteroid-induced pupariation. This developmental block is overcome by dietary 20-hydroxyecdysone, suggesting that a second locus in the region in addition to l(3)ecd may play a role in the regulation of late larval ecdysteroid levels.
Solar eclipse is an unique opportunity to study the lower ionospheric variabilities under a controlled perturbation when the solar ultraviolet and X-ray are temporally occulted by the lunar disk. Sub-ionospheric Very Low Frequency (VLF) radio signal displays the ionospheric response of solar eclipse by modulating its amplitude and phase. During the Total Solar Eclipse (TSE) on August 21, 2017 in North America, data was recorded by a number of receivers as presented in public archive. Out of these, two receiving stations YADA in McBaine and K5TD in Tulsa could procure a reasonable quality of noise free data where the signal amplitude was clearly modulated due to the eclipse. During the lunar occultation, a C3.0 solar flare occurred and the signal received from Tulsa manifested the effect of sudden ionization due to the flare. The VLF amplitude in Tulsa shows the effect which is generally understood by superimposing effects of both the solar eclipse and flare. However, the signal by YADA did not perturb by the solar flare, as the flaring * Corresponding author region was totally behind the lunar disk for the entire period. We numerically reproduced the observed signal amplitude variation at both the receiving locations by using Wait's two component D-region ionospheric model and the well-known Long Wavelength Propagation Capability (LWPC) code. The perturbed electron density for both the cases is computed which matches satisfactorily with the true ionospheric conditions.
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