Alkaline hydrolysis was performed on a series of different vegetable oils. The unsaponifiable lipid matter was extracted with ethyl ether, and the class of 4,4-desmethylsterols (sterols) plus the triterpene diols (diols) erythrodiol, uvaol, and betulinol were isolated by thin-layer chromatography. A validated method using the acetate derivatives of sterols instead of their silyl ethers is presented. The acetate derivatives were analyzed by high resolution gas chromatography (HRGC). Retention time, precision, recovery studies, and absolute response factors were calculated for these esters, and GC/mass spectrometric structure of the assigned retention times was confirmed for the sterols and triterpene diols.
The Food and Drug Administration has carried out a regulatory program since 1982 to control olive oil adulteration and mislabeling in the U.S. Analysis of imported and domestically packaged olive oil products and inspection of domestic packers have significantly reduced the presence of undeclared esterified olive oil in olive oil products. Undeclared esterified olive oil was present in 13% of olive oils examined in a 1985–86 survey, compared to 65% in a 1983–84 survey. Undeclared olive pomace oil and seed oils continue to require surveillance in a continuing effort to eliminate olive oil adulteration.
An in-laboratory validated method for identification and quantitation of sterols and triterpene diols was used to determine the relative composition of identifiable sterols and triterpene diols in 13 vegetable oils and quantitate these components in multiple samples of 6 vegetable oils: extra virgin olive, pomace olive, corn, cottonseed, canola, and evening primrose oils. Erythrodiol, a triterpene diol present in all olive oils, was also found in cottonseed oil.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.