Most multiple sclerosis (MS) patients develop over time a secondary progressive disease course, characterized histologically by axonal loss and atrophy. In early phases of the disease, focal inflammatory demyelination leads to functional impairment, but the mechanism of chronic progression in MS is still under debate. Reactive oxygen species generated by invading and resident central nervous system (CNS) macrophages have been implicated in mediating demyelination and axonal damage, but demyelination and neurodegeneration proceed even in the absence of obvious immune cell infiltration, during clinical recovery in chronic MS. Here, we employ intravital NAD(P)H fluorescence lifetime imaging to detect functional NADPH oxidases (NOX1–4, DUOX1, 2) and, thus, to identify the cellular source of oxidative stress in the CNS of mice affected by experimental autoimmune encephalomyelitis (EAE) in the remission phase of the disease. This directly affects neuronal function in vivo, as monitored by cellular calcium levels using intravital FRET–FLIM, providing a possible mechanism of disease progression in MS.
The association between attributional style, self-esteem, depressive symptoms, and general distress was examined to test hypotheses derived from a learned helplessness model and Weiner's (1979) attributional model of motivation. After answering questionnaires on depression, general distress, and self-esteem, 178 male and female undergraduate students were asked to make causal attributional ratings about 12 hypothetical events; 151 subjects also were asked to make diary ratings on 14 real events. Results revealed that attributional ratings were internally consistent across events; however, attributions about positive outcomes were either uncorrelated or positively correlated with attributions about negative outcomes, failing to support learned helplessness predictions that a single process underlies attributions about positive and negative events. As predicted, internal attributions for positive outcomes were primarily associated with high self-esteem. Only internal stable attributions for negative outcomes were related to depressive symptoms, consistent with Weiner's model. The pattern of correlation between attributions and general distress was essentially identical to that obtained with depressive symptoms. Attributions for real events were similar in their effects to ratings of hypothetical events.This document is copyrighted by the American Psychological Association or one of its allied publishers.This article is intended solely for the personal use of the individual user and is not to be disseminated broadly.
We report on the initial development and validation of the Living Donation Expectancies Questionnaire (LDEQ), designed to measure the expectations of living kidney donor candidates. Potential living donors (n=443) at two transplant centers were administered the LDEQ and other questionnaires, and their medical records were reviewed. Factor analysis provides support for six LDEQ scales: Interpersonal Benefit, Personal Growth, Spiritual Growth, Quid Pro Quo, Health Consequences, and Miscellaneous Consequences. All but one scale showed good internal consistency. Expected benefits of donation were associated with higher optimism and lower mental health; expected consequences of donation were associated with lower optimism and lower physical and mental health. More potential donors with relative or absolute contraindications had high Interpersonal Benefit (P<0.0001), Personal Growth (P<0.01), Quid Pro Quo (P<0.0001), and Health Consequences (P<0.0001) expectations. The LDEQ has promise in evaluating donor candidates' expectations.
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