Table l-Theobromine and caffeine levels in chocolate liquor Commercial chocolate products were analyzed for theobromine and caffeine content by High Performance Liquid Chromatography. Levels in 22 samples of chocolate liquor averaged 1.22% theobromine and 0.21% caffeine. Commercial cocoas contained, on the average, 1.89% theobromine and 0.21% caffeine. Sweet chocolate averaged 0.46% theobromine and 0.07% caffeine, while milk chocolate averaged 0.15% theobromine and 0.02% caffeine. Hot cocoa (chocolate) beverages averaged 65 mg of theobromine and 4 mg of caffeine per 5 ounce serving and chocolate milk prepared from a variety of cocoa-sugar mixes averaged 58 mg of theobromine and 5 mg of caffeine per 8 ounce serving. Theobromine and caffeine levels varied widely in individual samples within the product categories examined. Also, the ratio of theobromine to caffeine varied widely among different chocolate liquors ranging from a low of 2.5:1 to a high of 23.0: 1.
A high performance liquid chromatography (HPLC) method is described for the HPLC analysis of major phospholipids in soy lecithin. The method entails dissolving soy lecithin in chloroform prior to analysis. The HPLC determination uses a normal phase column and a mobile phase of acetonitrilemethanol‐H3PO4 with detection at 205 nm. The data presented illustrates that the method is rapid, accurate and precise for the determination of phospholipid in soy lecithin.
High performance liquid chromatography (HPLC) has been applied to the characterization and quantitation of the simple sugars (glucose, fructose, sucrose, maltose and lactose) present in a broad variety of food items, both fresh and processed. Following one of two simple extraction procedures, HPLC analysis is complete in 15 min. Recovery studies indicate good recovery from a variety of food matrices (94-104%). Relative standard deviations indicate excellent precision. The method permits an update of current carbohydrate literature both qualitatively and quantitatively.
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