SUMMARYThe reversal potential for the GABA-elicited hyperpolarization (EGABA) of the muscle bag cells of the parasitic nematode Ascaris suum is near to the equilibrium potential for chloride ions (ECI) indicating that this is a chloride-mediated event. The value of EGABA in the presence of different anions indicates the selectivity sequence of the GABA-operated chloride channel as SCN-> Br-, 1-> C103, NO3-, Cl-> C2H5COO-> CH3COO-,HCOO-,OHCH2CH2SO3H-, CH SO4-, BrO3 , So42-. The cut-off point in terms of relative hydrated size for permeation is between C103-and BrO3-suggesting that the diameter of the channel is between 0 29 and 0 33 nm. The selectivity sequence ofthe resting membrane chloride channels was indicated by the degree of muscle cell hyperpolarization upon addition of the anion to the bathing medium and was SCN-> NO3-> I, Br-, > CI03-> Cl-. These sequences are consistent with the anions interacting with a cationic site of low field strength at the selectivity determining portion of the channel for both the GABA-operated and the resting chloride channel.
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