Abstract. Hasanah R, Daningsih E, Titin. 2017. The analysis of nutrient and fiber content of banana (Musa paradisiaca) sold in Pontianak,. This study aimed to find out the effect of varieties of banana and market places to nutrients and fiber of bananas which were sold in Pontianak. Completely Randomized Design (CRD) Factorial model with main factors of varieties of banana (barangan, masak hijau, singapura), market places namely traditional market, fruit stores, and side road kiosk, and the combination of varieties and market places of banana. The variable tests were carbohydrate, glucose, fructose, sucrose, protein, lipid, vitamin C, crude fiber, water and ash content test. The result was processed with SAS application 6.12 version using ANOVA CRD Factorial and significances followed by LSD α=0.05. Result found varieties of banana affected on significantly to total carbohydrate, glucose, fructose, sucrose, vitamin C, lipid, and water but did not significantly affect on crude fiber, and ash. The market places gave no significant effect on total carbohydrate, glucose, fructose, sucrose, protein, vitamin C, crude fiber, water but gave significant affect on ash content. The combination between varieties and market places affected significantly on specific nutrient content. Barangan was good on total carbohydrate, vitamin C, and ash whilst masak hijau was highest on glucose, fructose, sucrose, and crude fiber. In addition, singapura was highest on protein, lipid and water.
The long-tailed macaques (Macaca fascicularis) is a primate species widely used in ecology, socio-economics, and biomedical. Long-tailed macaques have a high degree of adaptation to various types of environments. This species can be used and traded as long as it is the product of captivity. Over time, with increasing demand, there is a tendency to increase the number of long-tailed macaques catch quotas from nature and reduce the habitat of these primates in Indonesia. This demand causes the need for a conservation effort to increase the long-tailed macaques’ population outside their natural habitat. Tinjil Island is a semi-natural breeding place managed by the Primate Research Center (PRC), LPPM IPB University. We studied this area to observe the long-tailed macaques’ daily behaviour. We observed sexual behaviour with the ad-libitum method. Overall, the frequency of each stage of sexual behaviour carried out by the long-tailed macaques is genital inspect by 20% (for 12 seconds), male mounts by 9% (for 5 seconds); intromission by 44% (for 26 seconds), ejaculation success by 17% (for 10 seconds), vocalization by 7% (for 4 seconds), and running by 3% (for 1.6 seconds). Success in sexual behaviour is influenced by the sex age of primates, the period of lust, and food availability. This study is expected to provide information on sexual behaviour that can support reproductive success and successful reproductive management of long-tailed macaques in captivity.
Abstract. Hasanah R, Saepuloh U, Perwitasari-Farajallah D, Sinaga W, Hastuti Y, Sajuthi D. 2020. Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia. Biodiversitas 21: 2854-2861. Hylobates klossii is an endemic primate on the Mentawai Island, Indonesia that categorized as endangered species due to fragmentation, illegal hunting, and habitat loss. This study aimed to analyze the genetic characterization of H. klossii based on maternal and paternal lineages. The samples were collected from TSI (Bogor, Indonesia) cage consist of two males and two females (blood and fecal of four individuals) and Siberut Island, Mentawai, West Sumatra, Indonesia from one unknown gender (one individual fecal sample). PCR amplification was carried out on DNA and cDNA samples using specific primers for D-loop MtDNA and TSPY genes. The amplicon was analyzed with MEGA-7. The dimensional structure model of the TSPY protein was determined using I-TASSER and visualized with PyMol. The maternal analysis showed five samples consisted of four haplotypes revealed that Nam-nam and April were identic with 0% genetic distance. It’s revealed that Nam-nam and April were mother and son. The paternal analysis of two H. klossii males revealed that April was the closest relative to H. muelleri (99.56% identity) and Gou-gou to H. klossii (100% identity). Characterization of partial TSPY gene (990 bp) of April and Gou-Gou indicated four amino acids mutation, causing the shift in-amino acid structure position. The results assumed that April was a cross-breeding between H. klossii female and H. muelleri male. This genetic characterization is expected to support the conservation efforts through recommendations for good management in H. klossii preservation.
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