<p align="center"><strong><em>Abstract</em></strong></p><p><em> Pegagan (Centella asiatica (L.) Urban) is a traditional herbal plant that spread as it grows and in bloom througout the year. Pegagan is believed to be able to cure various type of diseases because it contains bioactive component that’s good for human body. Pegagan’s bioactive component that has anti-bacterial properties are saponin, flavonoid, </em><em>and </em><em>tanin. </em><em>T</em><em>his study was conducted with the purpose to determine the existence of antibacterial</em><em> activity in</em><em> ethanol extract of pegagan leaves by applying</em><em> it in</em><em> Disc Diffusion Test<sub> </sub>method against Escherichia coli and Staphylococcus aureus bacteria.</em></p><p><em> </em><em> Etanol extract</em><em> was obtained</em><em> by using maserasi method with 70% ethanol, separation of the active compound group which one of them is flavonoid using phytochemical screening, and the result was that the pegagan positively contains flavonoid, it was proven by the existence of a red marker on the tube. The result of active compund using methanol eluent: chloroform: glacial acetic acid produces 1 spot point on (Rf 0,5-0,7) </em><em>on </em><em>TLC.</em><em> This spot was used in antibacterial screening by bioatugraphy method and the activity was detected qualitatively.</em><em> Antibacterial activity was proved</em><em> by Disc Diffusion Test </em><em>which the results were ethanol extract on pegagan has inhibitory activity to E.coli and S. aureus.</em><em> This study can be concluded that ethanol extract </em><em>of </em><em>Centella asiatica has a MIC value of 3,200 µg / mL both in</em><em> E. Coli and</em><em> S. aureus. The diameter </em><em>of obstacles zone were</em><em> 0,06±0,05 mm to E.coli and 0,04±0,019 mm to S. aureus.</em><em></em></p><p align="right"><strong><em> </em></strong></p><p align="center"><strong><em>Abstrak</em></strong><em> </em></p><p><em>Centella asiatica (L.) Urban </em><em>(Pegagan)</em><em> </em><em>adalah</em><em> </em><em>spesies </em><em>tumbuhan herbal tradisional dengan karakteristik </em><em>tumbuh merambat dan</em><em> berbunga di sepanjang tahun.</em><em> </em><em>Komponen bioaktif yang dimiliki pegagan dalam beberapa aspek, dapat digunakan dalam pengobatan penyakit</em><em>. </em><em>Golongan-golongan senyawa</em><em> bioaktif pegagan yang memiliki daya antibakteri adalah saponin</em><em>, flavonoid, dan tanin</em><em>. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri ekstrak etanol daun pegagan dengan metode Disc Diffusion Test<sub> </sub>pada Escherichia coli maupun Staphylococcus aureus.</em></p><p><em> Ekstrak tanaman Pegagan</em><em> diperoleh dengan</em><em> maserasi dengan etanol 70%, pemisahan golongan senyawa aktif salah satunya flavonoid menggunakan skrining fitokimia,</em><em> yang secara kualitatif menunjukkan</em><em> </em><em>ekstrak tanaman</em><em> mengandung flavonoid.</em><em> </em><em>Hasil </em><em>Kromatografi Lapis Tipis (KLT) </em><em>senyawa aktif menggunakan eluen metanol: kloroform: asam asetat glasial menghasilkan 1 titik spot pada Rf 0,5-0,7 </em><em>digunakan dalam</em><em> penelusuran pengujian daya antibakteri dengan metode autobiografi dan menunjukkan adanya pembentukan zona bening. </em><em>Pada uji kuantitatif,</em><em> ekstrak etanol pegagan memiliki </em><em>nilai KHM 3.200 µg/mL </em><em>baik pada Eschericia coli maupun </em><em>Staphylococcus aureus. </em><em>N</em><em>ilai </em><em>Kadar Bunuh Minimum (</em><em>KBM</em><em>)</em><em> lebih dari 6.400 µg/mL</em><em> </em><em>a</em><em>ktivitas daya hambat dengan diameter 0,06±0,05</em><em> mm pada E.coli dan</em><em> 0,04±0,019</em><em> mm untuk S. aureus</em><em>.</em><em></em></p>
Wild mulberry (Morus nigra L.) is a kind of berries that has a high content of anthocyanin pigment. Anthocyanin is a natural pigment that has good biological activity so that widely be used as both food and drug ingredients. There are many studies conducted that have isolation anthocyanin from mulberry extract, but most of them used various expensive methods and the process included several steps that make them not cost-effective nor time-efficient. This research was conducted in order to do an isolation of anthocyanin from wild mulberry through a single step. The extraction of compounds was done by maceration and the isolation was done by thin layer chromatography method. The isolation product was identified with reagents, consisting of ferric chloride and sodium hydroxide, and with spectrophotometry methods, consisting of UV-Vis and infrared spectrophotometry. As result, this research was able to isolate anthocyanin from wild mulberry fruit by thin layer chromatography method. The identification with spectrophotometry methods indicated that the isolated compound hypothetically was anthocyanidin-3-O-rutinoside.
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