A study was performed to investigate the genomic variations in the shrimp farm
isolates of Vibrio alginolyticus and V. harveyi
when the isolates were subjected to environmental stress. Samples of shrimps, water
and sediment were collected from Southern Indian coastal shrimp farms. Vibrio
isolates were biochemically identified and confirmed using 16S rDNA and
gyrB gene specific PCR. The bacterial strains were genotyped by
PCR fingerprinting using GTG(5) and IS (Insertion Sequence) primers. Seven strains
each of V. alginolyticus and V. harveyi were
subjected to 10 passages through trypticase soya broth (TSB), which contained
different NaCl concentrations (3, 6 and 8%) and trypticase soya agar (TSA).
V. alginolyticus was also passaged through TSB with a 12% NaCl
concentration. PCR fingerprinting, which was performed on the strains that were
passaged through different salt concentrations, confirmed that V.
alginolyticus and V. harveyi could affect the genomic
variations, depending on the environmental conditions of the culture. The study
highlights the complex genotypic variations that occur in Vibrio
strains of tropical aquatic environment because of varied environmental conditions,
which result in genetic divergence and/or probable convergence. Such genetic
divergence and/or convergence can lead to the organismal adaptive variation, which
results in their ability to cause a productive infection in aquatic organisms or
generation of new strains.
White spot syndrome virus (WSSV) is a pathogen causing significant economic losses to shrimp aquaculture worldwide. Previously, five genome sequences of the virus from farmed shrimp (
Penaeus vannamei
and
Penaeus monodon
) in India were reported, all originating from farms located on the east coast of the country. Here, we report three new and distinct WSSV genome sequences, two from shrimp (
P. vannamei
) farmed on the west coast of India and the third from the east coast.
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