To compare the effects on serum lipoproteins of stearic acid, trans fatty acids, and dairy fat, 80 healthy subjects consumed a dairy fat-based (baseline) diet for 5 wk, then an experimental diet high in either trans fatty acids (8.7% of energy; n = 40) or stearic acid (9.3% of energy; n = 40) for another 5 wk. All diets provided 32.2-33.9% of energy as fat, 14.6-15.8% as saturated plus trans fatty acids, 11.4-12.5% as cis-monounsaturated fatty acids, 2.9-3.5% as polyunsaturated fatty acids, and 200-221 mg cholesterol/10 MJ. Compared with the dairy fat diet, stearic acid and trans fatty acids decreased serum total cholesterol concentrations similarly (by 13% and 12%, respectively, P < 0.001) but the trans fatty acid diet decreased HDL cholesterol (17%) and apolipoprotein (apo) A-I (15%) significantly more than did the stearic acid diet (11% and 12%, respectively). Stearic acid but not trans fatty acids reduced concentrations of LDL cholesterol and apo B significantly (P < 0.001). The trans fatty acid diet increased the ratio of LDL to HDL cholesterol (19%) and of apo B to apo A-I (16%) more than did the dairy fat diet (P < 0.001) but the stearic acid diet had no effect. Lipoprotein(a) concentrations increased with both experimental diets, significantly more with trans fatty acids (30%) than with stearic acid (10%). In conclusion, high amounts of trans fatty acids had more adverse effects on lipoproteins than did equal amounts of stearic acid and dairy fat. Stearic acid reduced LDL cholesterol, did not affect the ratio of LDL to HDL cholesterol, and increased lipoprotein(a), although to a lesser extent than did trans fatty acids. Dietary fats low in both saturated fatty acids and trans fatty acids should be favored.
The stability of encapsulated D-limonene, which was prepared by spray drying, was studied in view of the release characteristics and oxidation stability. Gum arabic, soybean water-soluble polysaccharide, or modified starch, blended with maltodextrin were used as the wall materials. The powders were stored under the conditions of 23-96% relative humidity at 50 degrees C. The release rate and the oxidation rate were closely related to the relative humidity. The relationship was not simple. Initially, the release rate and the oxidation rate increased with increasing water activity, but around the glass transition temperature, the rates decreased sharply to increase again at a further increase of water activity. The results could be explained by a change in the powder structure, where a glass capsule matrix was changed into rubbery state during storage.
Different possibilities for protein crosslinking are examined in this review, with special emphasis on enzymatic crosslinking and its impact on food structure. Among potential enzymes for protein crosslinking are transglutaminase (TG) and various oxidative enzymes. Crosslinking enzymes can be applied in cereal, dairy, meat, and fish processing to improve the texture of the product. Most of the current commercial applications are based on TG. The reaction mechanisms of the crosslinking enzymes differ, which in turn results in different technological properties.
Flaxseed oil was emulsified in whey protein isolate (WPI) and spray-dried. Powder characteristics and oxidative stability of oil at relative humidities (RH) from RH approximately 0% to RH 91% at 37 degrees C were analyzed. Oil droplets retained their forms in drying and reconstitution, but the original droplet size of the emulsion was not restored when the powder was dispersed in water. The particles seemed to be covered by a protein-rich surface layer as analyzed by electron spectroscopy for chemical analysis (ESCA). Oxidation of flaxseed oil dispersed in the WPI matrix was retarded from that of bulk oil but followed the same pattern as bulk oil with respect to humidity. A high rate of oxidation was found for both low and high humidity conditions. The lowest rate of oxidation as followed by peroxide values was found at RH 75%, a condition that is likely to diverge significantly from the monolayer moisture value. A weak baseline transition observed for the WPI matrix in a differential scanning calorimetry (DSC) thermogram suggested a glassy state of the matrix at all storage conditions. This was not consistent with the observed caking of the powder at RH 91%. Scanning electron microscopy (SEM) images revealed a considerable structural change in the WPI matrix in these conditions, which was suggested to be linked with a higher rate of oxygen transport. Possible mechanisms for oxygen transport in the whey protein matrix under variable RHs are discussed.
ABSTRA ABSTRA ABSTRA ABSTRA ABSTRACT CT CT CT CT: : : : : The effect of r The effect of r The effect of r The effect of r The effect of relativ elativ elativ elativ elative humidity (RH) (20 °C: RH 11%, 54%) on o e humidity (RH) (20 °C: RH 11%, 54%) on o e humidity (RH) (20 °C: RH 11%, 54%) on o e humidity (RH) (20 °C: RH 11%, 54%) on o e humidity (RH) (20 °C: RH 11%, 54%) on oxidativ xidativ xidativ xidativ xidative stability micr e stability micr e stability micr e stability micr e stability microencapsulated sea oencapsulated sea oencapsulated sea oencapsulated sea oencapsulated sea buckthorn seed oil was studied using bulk oil as a reference. Microcapsules were prepared by spray-drying using buckthorn seed oil was studied using bulk oil as a reference. Microcapsules were prepared by spray-drying using buckthorn seed oil was studied using bulk oil as a reference. Microcapsules were prepared by spray-drying using buckthorn seed oil was studied using bulk oil as a reference. Microcapsules were prepared by spray-drying using buckthorn seed oil was studied using bulk oil as a reference. Microcapsules were prepared by spray-drying using maltodextrin-gum arabic (MD/GA) and corn starch sodium octenyl succinate derivate (HiCap) as the wall materi-maltodextrin-gum arabic (MD/GA) and corn starch sodium octenyl succinate derivate (HiCap) as the wall materi-maltodextrin-gum arabic (MD/GA) and corn starch sodium octenyl succinate derivate (HiCap) as the wall materi-maltodextrin-gum arabic (MD/GA) and corn starch sodium octenyl succinate derivate (HiCap) as the wall materimaltodextrin-gum arabic (MD/GA) and corn starch sodium octenyl succinate derivate (HiCap) as the wall materials als als als als. . .. . The influence of the physical state of the wall mater The influence of the physical state of the wall mater The influence of the physical state of the wall mater The influence of the physical state of the wall mater The influence of the physical state of the wall material was also ev ial was also ev ial was also ev ial was also ev ial was also evaluated. U aluated. U aluated. U aluated. U aluated. Under dr nder dr nder dr nder dr nder dry conditions y conditions y conditions y conditions y conditions, the micr , the micr , the micr , the micr , the microenoen-oen-oenoencapsulated oils were most stable, but the oxidation of the bulk oil was accelerated. At 20 °C and at RH 11%, the capsulated oils were most stable, but the oxidation of the bulk oil was accelerated. At 20 °C and at RH 11%, the capsulated oils were most stable, but the oxidation of the bulk oil was accelerated. At 20 °C and at RH 11%, the capsulated oils were most stable, but the oxidation of the bulk oil was accelerated. At 20 °C and at RH 11%, the capsulated oils were most stable, but the oxidation of the bulk oil was accelerated. At 20 °C and at RH 11%, the peroxide value of the bulk oil exceeded 20 meq/kg within 1 wk. Microencapsulation prolonged the shelf-life of the oil peroxide value of the bulk oil exceeded 20 meq/kg within 1 wk. Microencapsulation pro...
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