The ALMA Survey of 70 µm dark High-mass clumps in Early Stages (ASHES) has been designed to systematically characterize the earliest stages and to constrain theories of high-mass star formation. A deep understanding of highmass star formation requires the study of the clustered mode, which is the most commonly found in nature. A total of 12 massive (>500 M ), cold (≤15 K), 3.6-70 µm dark prestellar clump candidates, embedded in infrared dark clouds (IRDCs), were carefully selected in the pilot survey to be observed with the Atacama Large Millimeter/sub-millimeter Array (ALMA). Exploiting the unique capabilities of ALMA, we have mosaiced each clump (∼1 arcmin 2 ) in dust continuum and line emission with the 12 m, 7 m, and Total Power arrays at 224 GHz (1.34 mm), resulting in ∼1. 2 angular resolution (∼4800 AU at the average source distance of 4 kpc). As the first paper of the series, we concentrate on the dust continuum emission to reveal the clump fragmentation. We have detected a total of 294 cores, from which 84 (29%) are categorized as protostellar based on outflow activity or "warm core" line emission. The remaining 210 (71%) are considered prestellar core candidates. The number of detected cores is independent of the mass sensitivity range of the observations and, on average, more massive clumps tend to form more cores. We find no correlation between the mass of the host clump and the most massive embedded core. We find a large population of low-mass (<1 M ) cores and no high-mass (>30 M ) prestellar cores. The most massive prestellar core has a mass of 11 M . From the prestellar core mass function, we derive a power law index of 1.17 ± 0.10, slightly shallower than the Salpeter index of 1.35. We have used the minimum spanning tree technique to characterize the separation between cores and their spatial distribution, and to derive mass segregation ratios. While there is a range of core masses and core separations detected in the sample, the mean separation and mean mass of cores per clump are well explained

A major goal of the Atacama Large Millimeter/submillimeter Array (ALMA) is to make accurate images with resolutions of tens of milliarcseconds, which at submillimeter (submm) wavelengths requires baselines up to ∼15 km. To develop and test this capability, a Long Baseline Campaign (LBC) was carried out from 2014 September to late November, culminating in end-to-end observations, calibrations, and imaging of selected Science Verification (SV) targets. This paper presents an overview of the campaign and its main results, including an investigation of the short-term coherence properties and systematic phase errors over the long baselines at the ALMA site, a summary of the SV targets and observations, and recommendations for science observing strategies at long baselines. Deep ALMA images of the quasar 3C 138 at 97 and 241 GHz are also compared to VLA 43 GHz results, demonstrating an agreement at a level of a few percent. As a result of the extensive program of LBC testing, the highly successful SV imaging at long baselines achieved angular resolutions as fine as 19 mas at ∼350 GHz. Observing with ALMA on baselines of up to 15 km is now possible, and opens up new parameter space for submm astronomy.

We have mapped the northern area (30 × 20 ) of a Local Group spiral galaxy M33 in 12 CO(J = 1-0) line with the 45 m telescope at the Nobeyama Radio Observatory. Along with Hα and Spitzer 24 μm data, we have investigated the relationship between the surface density of molecular gas mass and that of star formation rate (SFR) in an external galaxy (Kennicutt-Schmidt law) with the highest spatial resolution (∼80 pc) to date, which is comparable to scales of giant molecular clouds (GMCs). At positions where CO is significantly detected, the SFR surface density exhibits a wide range of over four orders of magnitude, from Σ SFR 10 −10 to ∼10 −6 M yr −1 pc −2 , whereas the Σ H 2 values are mostly within 10-40 M pc −2 . The surface density of gas and that of SFR correlate well at an ∼1 kpc resolution, but the correlation becomes looser with higher resolution and breaks down at GMC scales. The scatter of the Σ SFR -Σ H 2 relationship in the ∼80 pc resolution results from the variety of star-forming activity among GMCs, which is attributed to the various evolutionary stages of GMCs and to the drift of young clusters from their parent GMCs. This result shows that the Kennicutt-Schmidt law is valid only in scales larger than that of GMCs, when we average the spatial offset between GMCs and star-forming regions, and their various evolutionary stages.

Caloric restriction (CR) can extend longevity and modulate the features of obesity-related metabolic and vascular diseases. However, the functional roles of CR in regulation of revascularization in response to ischemia have not been examined. Here we investigated whether CR modulates vascular response by employing a murine hindlimb ischemia model. Wild-type (WT) mice were randomly divided into two groups that were fed either ad libitum (AL) or CR (65% of the diet consumption of AL). Four weeks later, mice were subjected to unilateral hindlimb ischemic surgery. Body weight of WT mice fed CR (CR-WT) was decreased by 26% compared with WT mice fed AL (AL-WT). Revascularization of ischemic hindlimb relative to the contralateral limb was accelerated in CR-WT compared with AL-WT as evaluated by laser Doppler blood flow and capillary density analyses. CR-WT mice had significantly higher plasma levels of the fat-derived hormone adiponectin compared with AL-WT mice. In contrast to WT mice, CR did not affect the revascularization of ischemic limbs of adiponectindeficient (APN-KO) mice. CR stimulated the phosphorylation of endothelial nitric-oxide synthase (eNOS) in the ischemic limbs of WT mice. CR increased plasma adiponectin levels in eNOS-KO mice but did not stimulate limb perfusion in this strain. CR-WT mice showed enhanced phosphorylation of AMP-activated protein kinase (AMPK) in ischemic muscle, and administration of AMPK inhibitor compound C abolished CR-induced increase in limb perfusion and eNOS phosphorylation in WT mice. Our observations indicate that CR can promote revascularization in response to tissue ischemia via an AMPK-eNOS-dependent mechanism that is mediated by adiponectin.Obesity is closely associated with the development of metabolic syndrome and type 2 diabetes (1), which contribute to microvascular rarefaction and impaired collateral vessel growth under ischemic conditions (2-4). These conditions lead to increased vulnerability to ischemic injury and impaired wound healing and promote the progression of cardiovascular diseases. Thus, therapeutic approaches that enhance revascularization could be beneficial for ischemic vascular disease.Caloric restriction (CR) 4 has been shown to extend the life span of multiple species by retarding the aging process (5). In obese subjects CR has been shown to reduce visceral fat accumulation and also decrease body weight (6). CR have also been reported to lead to a reduction of hyperglycemia and hyperlipidemia that are major risk factors for ischemic heart diseases (7-12). A number of experimental studies have shown that CR attenuates atherosclerotic lesion formation (11), pathological cardiac hypertrophy (13), and ischemia-induced myocardial damage (14). These findings suggest that CR counteracts the unfavorable features of obese complications. However, the consequences of CR on vascular responses to tissue ischemia have not been examined.Adipose tissue secretes a variety of bioactive molecules, referred to as adipokines, that directly affect obesity-linked disord...