Hristov, A. N., Hanigan, M., Cole, A., Todd, R., McAllister T. A., Ndegwa, P. and Rotz, A. 2011. Review: Ammonia emissions from dairy farms and beef feedlots. Can. J. Anim. Sci. 91: 1–35. Ammonia emitted from animal feeding operations is an environmental and human health hazard, contributing to eutrophication of surface waters and nitrate contamination of ground waters, soil acidity, and fine particulate matter formation. It may also contribute to global warming through nitrous oxide formation. Along with these societal concerns, ammonia emission is a net loss of manure fertilizer value to the producer. A significant portion of cattle manure nitrogen, primarily from urinary urea, is converted to ammonium and eventually lost to the atmosphere as ammonia. Determining ammonia emissions from cattle operations is complicated by the multifaceted nature of the factors regulating ammonia volatilization, such as manure management, ambient temperature, wind speed, and manure composition and pH. Approaches to quantify ammonia emissions include micrometeorological methods, mass balance accounting and enclosures. Each method has its advantages, disadvantages and appropriate application. It is also of interest to determine the ammonia emitting potential of manure (AEP) independent of environmental factors. The ratio of nitrogen to non-volatile minerals (phosphorus, potassium, ash) or nitrogen isotopes ratio in manure has been suggested as a useful indicator of AEP. Existing data on ammonia emission factors and flux rates are extremely variable. For dairy farms, emission factors from 0.82 to 250 g ammonia per cow per day have been reported, with an average of 59 g per cow per day (n=31). Ammonia flux rates for dairy farms averaged 1.03 g m−2 h−1 (n=24). Ammonia losses are significantly greater from beef feedlots, where emission factors average 119 g per animal per day (n=9) with values as high as 280 g per animal per day. Ammonia flux rate for beef feedlots averaged 0.174 g m−2 h−1 (n=12). Using nitrogen mass balance approaches, daily ammonia nitrogen losses of 25 to 50% of the nitrogen excreted in manure have been estimated for dairy cows and feedlot cattle. Practices to mitigate ammonia emissions include reducing excreted N (particularly urinary N), acidifying ammonia sources, or binding ammonium to a substrate. Reducing crude protein concentration in cattle diets and ruminal protein degradability are powerful tools for reducing N excretion, AEP, and whole-farm ammonia emissions. Reducing dietary protein can also benefit the producer by reducing feed cost. These interventions, however, have to be balanced with the risk of lost production. Manure treatment techniques that reduce volatile N species (e.g., urease inhibition, pH reduction, nitrification-denitrification) are also effective for mitigating ammonia emissions. Another option for reducing ammonia emissions is capture and treatment of released ammonia. Examples in the latter category include biofilters, permeable and impermeable covers, and manure incorporation into the soil for crop or pasture production. Process-level simulation of ammonia formation and emission provides a useful tool for estimating emissions over a wide range of production practices and evaluating the potential benefits of mitigation strategies. Reducing ammonia emissions from dairy and beef cattle operations is critical to achieving environmentally sustainable animal production that will benefit producers and society at large.

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Background The colposcopy-directed punch biopsy is widely used in the management of women with abnormal cervical cytology; however, its accuracy compared with definitive histology from an excision biopsy is not well established.Objectives To assess the accuracy of the colposcopy-directed punch biopsy to diagnose high-grade cervical intraepithelial neoplasia (CIN) by performing a systematic review and meta-analysis.Search strategy A systematic search of MEDLINE, EMBASE and the Cochrane Library was performed.Selection criteria Articles that compared the colposcopically directed cervical punch biopsy with definitive histology from an excisional cervical biopsy or hysterectomy.Data collection and analysis Random effects and hierarchical summary receiver operating characteristic regression models were used to compute the pooled sensitivity and specificity applying different test cut-offs for outcomes of high-grade CIN.Main results Thirty-two papers comprising 7873 paired punch/ definitive histology results were identified. The pooled sensitivity for a punch biopsy defined as test cut-off CIN1+ to diagnose CIN2+ disease was 91.3% (95% CI 85.3-94.9%) and the specificity was 24.6% (95% CI 16.0-35.9%). In most of the studies, the majority of enrolled women had positive punch biopsies. Pooling of the four studies where the excision biopsy was performed immediately after the punch biopsy, and where the rate of positive punch biopsies was considerably lower, yielded a sensitivity of 81.4% and specificity of 63.3%.Author's conclusion The observed high sensitivity of the punch biopsy derived from all studies is probably the result of verification bias.

Emissions of ammonia, as well as other gases and particulates, to the atmosphere are a growing concern of livestock producers, the general public, and regulators. The concentration and ruminal degradability of CP in beef cattle diets may affect urinary and fecal excretion of N and thus may affect ammonia emissions from beef cattle feed yards. To determine the effects of dietary CP concentration and degradability on potential ammonia emissions, 54 steers were randomly assigned to nine dietary treatments in a 3 × 3 factorial arrangement of treatments. Treatments consisted of three dietary CP concentrations (11.5, 13, and 14.5%) and three supplemental urea:cottonseed meal ratios (100:0, 50:50, and 0:100 of supplemental N). Steers were confined to tie stalls, and feces and urine excreted were collected and frozen after approximately 30, 75, and 120 d on feed. One percent of daily urine and feces

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Reliable estimates of N excretion in the urine and feces of beef cattle are essential for developing cost-effective and environmentally sound nutrient management plans. A meta-analysis dataset was compiled that included data for starting live BW, DMI, N intake, dietary CP and RDP concentrations, urine N excretion, and feces N excretion. The data were taken from 12 individual feeding trials that included N balance data, and represented a total of 47 different dietary treatments and 255 animals. Correlation analysis was used to determine the animal and dietary parameters that were most closely related to N excretion in urine and feces by beef cattle. A multivariate mixed modeling approach was used to develop empirical equations to predict excretion of urine N, feces N, and the partitioning of total N excretion between urine and feces, as a function of N intake and the concentration of dietary CP. Univariate, regression, and mean difference comparisons indicated 46 to 95% agreement between observed and predicted values for the developed equations. Evaluation of the equations with an independent dataset taken from 6 studies, and 2 random subsets of the meta-analysis dataset showed moderate agreement (P < 0.05, r(2) = 0.34 to 0.86) for urine N excretion as a function of both N intake and %CP, and the partitioning of total N excretion into urine as a function of %CP. There was less agreement between predictions and observations for feces N excretion as a function of %CP (r(2) = 0.003 to 0.14) than N intake (r(2) = 0.52 to 0.75), indicating that %CP is not a good predictor of fecal N excretion. The empirical equations provide a simple tool that, if used with caution, could predict N excretion characteristics for a wide range of dietary and animal characteristics and could improve ammonia emissions estimates by process-based models.