We present the design and construction of a versatile, open frame inverted microscope system for wide-field fluorescence and single molecule imaging. The microscope chassis and modular design allow for customization, expansion, and experimental flexibility. We present two components which are included with the microscope which extend its basic capabilities and together create a powerful microscopy system: A Convex Lens-induced Confinement device provides the system with single-molecule imaging capabilities, and a two-color imaging system provides the option of imaging multiple molecular species simultaneously. The flexibility of the open-framed chassis combined with accessible single-molecule, multi-species imaging technology supports a wide range of new measurements in the health, nanotechnology, and materials science research sectors.
Ultrasonic diffraction pattern at a crack tip and crack depth sizing based on the diffraction are studied by using laser-generated bulk waves. Directivity patterns oriented essentially along the normal for the diffracted longitudinal wave and essentially at 45 (deg) for the diffracted shear wave for any incident angles is obtained. A laser-ultrasonic system combined with time-offlight (TOFD) analysis is produced and demonstrates accurate crack sizing on an artificial slot having a variable depth from 0 mm to 10 mm. An improvement of signal-to-noise ratio by split spectrum processing (SSP) is also suggested.
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