Abstract— —Continuous cell lines, primary cell cultures derived from embryonic CNS, and homogenates made from adult and embryonic CNS were compared with respect to their lipid pattern and their ability to bind 125I‐labelled tetanus toxin. In parallel experiments de novo synthesis of gangliosides in the cell lines was studied, using [14C]glucosamine as precursor. Of the total lipid only gangliosides were specifically labelled by [14C]glucosamine. The patterns of the de novo synthesized gangliosides corresponded to those present in the respective cells.
Pronounced binding of 125I‐labelled toxin was only detectable in tissues containing long‐chain gangliosides (ganglioside C which represents GDIb and GTI).
Accordingly, hybrid (neuroblastoma x glioma) cells, due to their lack of long‐chain gangliosides, bound just‐discernible amounts of labelled toxin. When previously exposed to gangliosides, their binding of tetanus toxin tremendously increased.
It was concluded that only the long‐chain gangliosides in the neuronal cells are functionally involved in the binding of the tetanus toxin and that these acceptors of tetanus toxin can be transplanted.
capsid protein of FPV and that against annexin 33 kDa were obtained from Drs Becht and Lim as previously described [2]. Anti-annexin V Abstract Influenza viruses bind to annexin V, a widely spread antiserum was produced in a rabbit by immunizing with 50 ~g of non-glycosylated phospholipid-binding protein. Externally added annexin V in the presence of Freund's adjuvant at monthly intervals phospholipids as well as antiserum against this protein specifiover a period of 2 months. The highest antibody titer obtained was cally inhibit infection of these viruses in cell cultures. We about 1:500 as determined by ELISA. Immunostaining of the virus conclude that annexin V plays an important role in the entry of specific nucleocapsid protein (NP) in the nuclei of infected cells was these viruses, performed 2 h after virus infection.
Phospholipids
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