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A B S T R Anormal granulocytes, appear to contain mainly TC III.No TC II was present in any of the sonicates. The general practice in most laboratories has been to determine serum UBBC. Because in vitro increments of up to 119% were found to occur in serum, this practice should be replaced by collection using methods that prevent such increments. Blood collected in EDTA-47 mM NaF had a stable, reproducible UBBC with no significant in vitro increment with time.EDTA-NaF UBBC was 640±168 (range 380-921 pg Bra bound/ml plasma) for 12 normal adult men and 809±232 (range 505-1208) for 10 normal adult women.It presumably approximates circulating UBBC and is substantially below the serum UBBC mean of 935±262 (range 611-1506 for the same 12 men) and 1273±355 (range 811-2306 for the same 10 women).

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