We describe here a system for the rapid identification, assay
development, and characterization of gene-based single nucleotide
polymorphisms (SNPs). This system couples informatics tools that mine
candidate SNPs from public expressed sequence tag resources and
automatically designs assay reagents with detection by a chip-based
matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry platform. As a proof of concept of this system, a
genomewide collection of reagents for 9,115 gene-based SNP genetic
markers was rapidly developed and validated. These data provide
preliminary insights into patterns of polymorphism in a genomewide
collection of gene-based polymorphisms.
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