Estimating contributions by root respiration and root litter to total soil respiration is difficult owing to problems in measuring each component separately. In a mixed hardwood forest in Massachusetts, we added or removed aboveground litter and terminated live root activity through construction of trenches and root barriers to determine the contribution of aboveground litter, belowground litter, and root respiration to total soil respiration. Annual soil respiration at control plots, measured by the soda-lime technique, was 371 g C•m−2-year−1. We used aboveground litter inputs (138 g C•m−2year−1) and differences in carbon dioxide effluxes among treatment plots to calculate contributions to total soil respiration by live root respiration (33%) and by organic matter derived from aboveground (37%) and belowground (30%) litter. Newly deposited aboveground litter contributed 31% of the carbon dioxide emitted by total aboveground litter. This estimate is consistent with values published in litter decomposition studies. Nearly two thirds of soil respiration in this forest can be attributed to root activity, comparable with a previous study suggesting that live root respiration plus decomposition of root litter contributes 70–80% of total soil respiration across a wide range of forests.
Reported in this paper are foliar chemistry, tree growth (above-and belowground), soil chemistry, nitrogen cycling (net mineralization and nitrification) and soil N 2 O flux responses to the first 6 yr of chronic nitrogen amendments at the Harvard Forest (Massachusetts, USA). A 70-yr-old red pine (Pinus resinosa Ait.) stand and a 50-yr-old mixed hardwood stand received control, low nitrogen (50 kg·ha Ϫ1 ·yr Ϫ1 ), high nitrogen (150 kg·ha Ϫ1 ·yr Ϫ1 ), and low nitrogen plus sulfur treatments, with additions occurring in six equal doses over the growing season as NH 4 NO 3 and Na 2 SO 4 . Foliar N concentrations increased up to 25% in the hardwood stand and 67% in the pines, and there was no apparent decrease of N retranslocation due to fertilization. Wood production increased in the hardwood stand in response to fertilization but decreased in the pine stand. Fine-root nitrogen concentrations increased with N additions, and fine roots were a significant sink for added nitrogen. Nitrate leaching losses increased continuously over the 6-yr period in the treated pine stands but remained insignificant in the hardwoods. Annual net N mineralization increased substantially in response to treatments in both stands but declined in the pine high-N plot by the end of year six. Net nitrification increased from 17% of net mineralization in 1988 to 51% in 1993 for the pine high-N plot. Only a slight increase in net nitrification was measured in the hardwood stand, and only in 1993. Extractable NH 4 was consistently higher in treated plots than in controls in both stands, where extractable NO 3 was higher than controls only in the treated pine plots. Soil extracts yielded Ͻ1.5 kg/ha of NO 3 -N for all plots in the hardwood stand throughout the experiment. Effluxes of N 2 O were consistently greater in the pine high-N plot than in the pine control plot, but there were no observed large-scale increases in N 2 O emissions immediately following fertilizer application. Calculated nitrogen budgets for the first 6 yr showed extremely high N retention (85-99%). Of the retained N, 50-83% appears to be in the long-term, recalcitrant soil pool. The relative importance of biotic and abiotic mechanisms of N incorporation into soils remains uncertain. Size, kinetics, and uptake capacity of this soil pool are critical and largely unknown factors determining ecosystem response to increased N loading and may be related to land-use history.
Over the past 6 years (1988–1993), we have examined the effects of soil temperature, soil moisture, site fertility, and nitrogen fertilization on the consumption of atmospheric CH4 by temperate forest soils located at the Harvard Forest in Petersham, Massachusetts. We found that soil temperature is an important controller of CH4 consumption at temperatures between −5° and 10°C but had no effect on CH4 consumption at temperatures between 10° and 20°C. Soil moisture exerts strong control on CH4 consumption over a range of 60 to 100% water‐filled pore space (% WFPS). As moisture increased from 60 to 100% WFPS, CH4 consumption decreased from 0.1 to 0 mg CH4‐C m−2 h−1 because of gas transport limitations. At 20 to 60% WFPS, site fertility was a strong controller of CH4 consumption. High‐fertility sites had 2 to 3 times greater CH4 consumption rates than low‐fertility sites. Nitrogen‐fertilized soils (50 and 150 kg NH4NO3‐N ha−1 yr−1 ) had annually averaged CH4 consumption rates that were 15 to 64% lower than annually averaged CH4 consumption by control soils. The decrease in CH4 consumption was related to both the years of application and quantity of nitrogen fertilizer added to these soils.
Sequential density fractionation separated soil particles into ''light'' predominantly mineral-free organic matter vs. increasingly ''heavy'' organo-mineral particles in four soils of widely differing mineralogy. With increasing particle density C concentration decreased, implying that the soil organic matter (OM) accumulations were thinner. With thinner accumulations we saw evidence for both an increase in 14 C-based mean residence time (MRT) of the OM and a shift from plant to microbial origin.Evidence for the latter included: (1) a decrease in C/N, (2) a decrease in lignin phenols and an increase in their oxidation state, and (3) an increase in d 13 C and d 15 N. Although bulk-soil OM levels varied substantially across the four soils, trends in OM composition and MRT across the density fractions were similar. In the intermediate density fractions (*1.8-2.6 g cm -3 ), most of the reactive sites available for interaction with organic molecules were provided by aluminosilicate clays, and OM characteristics were consistent with a layered mode of OM accumulation. With increasing density (lower OM loading) within this range, OM showed evidence of an increasingly microbial origin. We hypothesize that this microbially derived OM was young at the time of attachment to the mineral surfaces but that it persisted due to both binding with mineral surfaces and protection beneath layers of younger, less microbially processed C. As a result of these processes, the OM increased in MRT, oxidation state, and degree of microbial processing in the sequentially denser intermediate fractions. Thus mineral surface chemistry is assumed to play little role in determining
In an old growth coniferous forest located in the central Cascade Mountains, Oregon, we added or removed aboveground litter and terminated live root activity by trenching to determine sources of soil respiration. Annual soil efflux from control plots ranged from 727 g C m À2 year À1 in 2002 to 841 g C m À2 year À1 in 2003. We used aboveground litter inputs (149.6 g C m À2 year À1 ) and differences in soil CO 2 effluxes among treatment plots to calculate contributions to total soil efflux by roots and associated rhizosphere organisms and by heterotrophic decomposition of organic matter derived from aboveground and belowground litter. On average, root and rhizospheric respiration (R r ) contributed 23%, aboveground litter decomposition contributed 19%, and belowground litter decomposition contributed 58% to total soil CO 2 efflux, respectively. These values fall within the range of values reported elsewhere, although our estimate of belowground litter contribution is higher than many published estimates, which we argue is a reflection of the high degree of mycorrhizal association and low nutrient status of this ecosystem. Additionally, we found that measured fluxes from plots with doubled needle litter led to an additional 186 g C m À2 year À1 beyond that expected based on the amount of additional carbon added; this represents a priming effect of 187%, or a 34% increase in the total carbon flux from the plots. This finding has strong implications for soil C storage, showing that it is inaccurate to assume that increases in net primary productivity will translate simply and directly into additional belowground storage.
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